Mazure G, Fernandes T, McCarthy D A, Macey M, Perry J D, Taub N A, Dumonde D C, Brown K A
Department of Immunology, UMDS, London, UK.
Int Arch Allergy Immunol. 1995 Nov;108(3):211-23. doi: 10.1159/000237156.
Monocytes from 17 patients with rheumatoid arthritis (RA) were more adherent than monocytes from 17 control patients to monolayers of pig aortic endothelium irrespective of whether sera was included (median 27-34% increase; p = 0.002) or omitted (median 27% increase; p = 0.022) from the culture media. When human umbilical vein endothelial cells were used as the adherence substrate, rheumatoid monocytes from an additional 21 patients demonstrated a median 31% (p = 0.004) and 20% increase (p = 0.004) in adhesion when compared with monocytes from 21 normal healthy subjects in the absence and presence of autologous sera, respectively. Activation of control monocytes with muramyl dipeptide or treatment with RA sera increased their attachment to endothelium (mean 34 +/- 14% increase; p < 0.001). The expression of the adhesion molecules CD11b (p < 0.005), CD18 (p < 0.005), CD62L (p = 0.01) was enhanced on rheumatoid monocytes, but antibody-blocking studies suggested that CD18 and CD62L were not responsible for the augmented binding of the rheumatoid cells. A subpopulation of rheumatoid monocytes possessed a very low net negative surface charge, a property that favours binding to vessel walls. We propose that many rheumatoid monocytes are predisposed for sheer-resistant adhesion to vascular endothelium.
17例类风湿关节炎（RA）患者的单核细胞比17例对照患者的单核细胞对猪主动脉内皮细胞单层的黏附性更强，无论培养基中是否含有血清（中位数增加27%-34%；p = 0.002）或不含血清（中位数增加27%；p = 0.022）。当使用人脐静脉内皮细胞作为黏附底物时，另外21例患者的类风湿单核细胞在无自体血清和有自体血清的情况下，与21例正常健康受试者的单核细胞相比，黏附性分别中位数增加31%（p = 0.004）和20%（p = 0.004）。用胞壁酰二肽激活对照单核细胞或用RA血清处理可增加其对内皮细胞的黏附（平均增加34±14%；p < 0.001）。类风湿单核细胞上黏附分子CD11b（p < 0.005）、CD18（p < 0.005）、CD62L（p = 0.01）的表达增强，但抗体阻断研究表明CD18和CD62L与类风湿细胞结合增加无关。类风湿单核细胞的一个亚群表面净负电荷极低，这一特性有利于与血管壁结合。我们提出，许多类风湿单核细胞易于对血管内皮细胞产生抗剪切力的黏附。
