采用液相色谱-荧光检测法测定利血平原料药中阿吗碱的含量。
Determination of ajmalicine in reserpine raw materials by liquid chromatography with fluorescence detection.
作者信息
Cieri U R
机构信息
U.S. Food and Drug Administration, Philadelphia, PA 19106, USA.
出版信息
J AOAC Int. 1995 Jul-Aug;78(4):944-5.
A method is presented for determination of ajmalicine in reserpine raw materials by liquid chromatography (LC) with fluorescence detection. The sample is dissolved in a very small volume of chloroform, and the resulting solution is diluted with methanol. The reference solution of ajmalicine is prepared directly in methanol. For LC, a 30 cm long normal-phase column is used. The mobile phase is methanol containing a small volume of an aqueous solution of 1-pentanesulfonic acid, sodium salt. Detection is by fluorescence with excitation at 280 nm and emission at 360 nm. In 3 samples, the ajmalicine contents ranged from 1.2 to 1.9%.
提出了一种采用带荧光检测的液相色谱法（LC）测定利血平原料药中阿吗碱含量的方法。将样品溶解于极少量氯仿中，所得溶液用甲醇稀释。阿吗碱对照溶液直接用甲醇配制。对于液相色谱，使用一根30 cm长的正相柱。流动相为含有少量1-戊烷磺酸钠盐水溶液的甲醇。采用荧光检测，激发波长为280 nm，发射波长为360 nm。在3个样品中，阿吗碱含量范围为1.2%至1.9%。
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