Xue G, Yuan S
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing.
Zhongguo Zhong Yao Za Zhi. 1996 Sep;21(9):554-5, 576.
A HPLC method for separating and preparing indole alkaloids is described. HPLC conditions for analysis: BIO-RAD series 700 HPLC, model 700 data station, UV: model 1749 UV-VIS monitor, column: BIO-RAD Hi-pore RP318, 250 mm x 10 mm, mobile phase: 80% methanol-H2O(gradient), flow rate: 1.5 ml/min, detection wavelength: 254 nm. On the basis of spectral (1HNMR, 13CNMR, H-H COSY, MS, DEPT) and chemical evidence, the structures of two compounds were elucidated as beta-yohimbine (yohimban-16-carboxylic acid-17-hydroxy methylester (3 alpha, 16 alpha, 17 beta)) and ajmalicine (oxayohimban-16-carboxylic acid-16,17-didehydro-19-ethyl methyl ester (19 alpha)).
描述了一种用于分离和制备吲哚生物碱的高效液相色谱（HPLC）方法。分析的HPLC条件：BIO-RAD系列700高效液相色谱仪、700型数据处理站、UV：1749型紫外可见监测器、色谱柱：BIO-RAD Hi-pore RP318，250 mm×10 mm、流动相：80%甲醇-水（梯度）、流速：1.5 ml/min、检测波长：254 nm。基于光谱（1H NMR、13C NMR、H-H COSY、MS、DEPT）和化学证据，确定两种化合物的结构分别为β-育亨宾（育亨烷-16-羧酸-17-羟基甲酯（3α，16α，17β））和阿吗碱（氧化育亨烷-16-羧酸-16,17-二脱氢-19-乙基甲酯（19α））。
