用于米舍尔-达顿系统的无血清培养基。
A serum-free medium for the Mishell-Dutton system.
作者信息
Burger M
出版信息
Eur J Immunol. 1977 Dec;7(12):906-8. doi: 10.1002/eji.1830071216.
PMID:75805
Abstract
A serum-free medium which provides high recoveries of plaque-forming cells in the primary immune response to sheep erythrocytes is described. In this medium the serum was replaced by heated fetuin and by excess of nonessential amino acids. Glycopeptides, obtained by excessive pronase treatment of fetuin were partially capable of replacing the nonhydrolyzed fetuin.
摘要
本文描述了一种无血清培养基，该培养基在对绵羊红细胞的初次免疫应答中能使空斑形成细胞获得高回收率。在这种培养基中，血清被加热的胎球蛋白和过量的非必需氨基酸所取代。通过用过量的链霉蛋白酶处理胎球蛋白获得的糖肽部分能够替代未水解的胎球蛋白。
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