在无血清及其蛋白质的情况下，使用二氧化硅和2-巯基乙醇对小鼠脾细胞培养物进行免疫接种。
Immunization of mouse spleen cell cultures in the absence of serum and its proteins using SiO2 and 2-mercaptoethanol.
作者信息
Burger M
出版信息
Immunology. 1982 Feb;45(2):381-5.
Abstract
High recoveries of plaque-forming cells were obtained in a serum-free medium using 2-mercaptoethanol and SiO2. Under such conditions an increase in the number of plaque-forming cells occurred between the fourth and fifth day of incubation, as compared with cultures with foetal calf serum. The addition of glutathione to the medium had a stimulating effect, it was however not absolutely necessary for a positive response. The recovery of plaque-forming cells was strongly influenced by an interdependence of cell density and amount of SiO2 gel particles.
摘要
在使用2-巯基乙醇和二氧化硅的无血清培养基中可获得较高的蚀斑形成细胞回收率。在这种条件下，与含有胎牛血清的培养物相比，蚀斑形成细胞的数量在培养的第4天到第5天之间有所增加。向培养基中添加谷胱甘肽有刺激作用，但对于阳性反应来说并非绝对必要。蚀斑形成细胞的回收率受到细胞密度和二氧化硅凝胶颗粒量之间相互依存关系的强烈影响。
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本文引用的文献
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The capacity of fetal calf serum to support a primary antibody response in vitro is determined, in part, by its reduced glutathione content.胎牛血清在体外支持初次抗体反应的能力，部分取决于其还原型谷胱甘肽的含量。
Cell Immunol. 1980 Aug 1;53(2):325-32. doi: 10.1016/0008-8749(80)90332-9.
2
The replacement of serum by SiO2 (aerosil) and 2-mercaptoethanol or diethanoldisulfide in the immunization and stimulation of spleen cell cultures.在脾细胞培养的免疫和刺激过程中，用二氧化硅（气相法二氧化硅）、2-巯基乙醇或二乙二硫取代血清。
FEBS Lett. 1980 Sep 8;118(2):212-4. doi: 10.1016/0014-5793(80)80221-3.
3
Adjuvant effects of amorphous silica and of aluminium hydroxide on IgE and IgG1 antibody production in different inbred mouse strains.无定形二氧化硅和氢氧化铝对不同近交系小鼠IgE和IgG1抗体产生的佐剂效应。
Int Arch Allergy Appl Immunol. 1980;61(3):253-8. doi: 10.1159/000232443.
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Eur J Immunol. 1980 Oct;10(10):796-8. doi: 10.1002/eji.1830101014.
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Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
6
Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.在脂多糖反应性B淋巴细胞培养物中用白蛋白、转铁蛋白和大豆脂质完全替代血清。
J Exp Med. 1978 Mar 1;147(3):923-33. doi: 10.1084/jem.147.3.923.
7
A serum-free medium for the Mishell-Dutton system.用于米舍尔-达顿系统的无血清培养基。
Eur J Immunol. 1977 Dec;7(12):906-8. doi: 10.1002/eji.1830071216.
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