Allen M J, Collick A, Jeffreys A J
Department of Genetics, University of Leicester, UK.
PCR Methods Appl. 1994 Oct;4(2):71-5. doi: 10.1101/gr.4.2.71.
Vectorette PCR permits the specific amplification of DNA segments flanking a known DNA sequence. It enables the application of the PCR where sequence information is only available for one primer site. We now show that vectorette PCR can be used for the systematic mapping and retrieval of transgene flanking DNA. We also show that the sequence of large vectorette PCR fragments can be obtained without cloning, by the production of subvectorette fragments.
载体引物PCR可特异性扩增已知DNA序列侧翼的DNA片段。它使得在仅一个引物位点有序列信息的情况下也能应用PCR。我们现在表明载体引物PCR可用于转基因侧翼DNA的系统定位和检索。我们还表明，通过产生亚载体引物片段，无需克隆就能获得大型载体引物PCR片段的序列。
