链霉菌噬菌体phiBT1的整合位点及位点特异性整合载体的构建
Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.
作者信息
Gregory Matthew A, Till Rob, Smith Margaret C M
机构信息
Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom.
出版信息
J Bacteriol. 2003 Sep;185(17):5320-3. doi: 10.1128/JB.185.17.5320-5323.2003.
Abstract
Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.
摘要
尽管链霉菌温和噬菌体phiC31和phiBT1的基因组之间存在广泛的相似性，但attP-int位点的保守性较差。在这里，我们证明phiBT1整合到与phiC31不同的附着位点。phiBT1 attB位于编码一种79个氨基酸的假定整合膜蛋白的SCO4848内。基于phiBT1整合酶的整合载体显示具有广泛的宿主范围，并且与基于phiC31 attP-int位点的载体完全兼容。
相似文献
1
Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.链霉菌噬菌体phiBT1的整合位点及位点特异性整合载体的构建
J Bacteriol. 2003 Sep;185(17):5320-3. doi: 10.1128/JB.185.17.5320-5323.2003.
2
Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by Streptomyces phage phiC31 integrase.通过链霉菌噬菌体 phiC31 整合酶鉴定有利于转基因整合和表达的猪细胞中的伪 attP 位点。
BMC Mol Biol. 2013 Sep 8;14:20. doi: 10.1186/1471-2199-14-20.
3
Site-specific transgene integration in the human genome catalyzed by phiBT1 phage integrase.由phiBT1噬菌体整合酶催化的人基因组中位点特异性转基因整合。
Hum Gene Ther. 2008 Feb;19(2):143-51. doi: 10.1089/hum.2007.110.
4
Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.一种新型链霉菌噬菌体ϕJoe介导的基因组整合与切除
Appl Environ Microbiol. 2017 Feb 15;83(5). doi: 10.1128/AEM.02767-16. Print 2017 Mar 1.
5
Site-specific integration of Streptomyces PhiC31 integrase-based vectors in the chromosome of Rhodococcus equi.基于链霉菌PhiC31整合酶的载体在马红球菌染色体中的位点特异性整合。
FEMS Microbiol Lett. 2008 Oct;287(1):63-8. doi: 10.1111/j.1574-6968.2008.01298.x. Epub 2008 Aug 1.
6
Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase.基于链霉菌噬菌体phiBT1整合酶的高效体外位点特异性重组系统。
J Bacteriol. 2008 Oct;190(19):6392-7. doi: 10.1128/JB.00777-08. Epub 2008 Aug 8.
7
[Progress of φC31 integrase system in site-specific integration].[φC31整合酶系统在位点特异性整合中的研究进展]
Yi Chuan. 2011 Jun;33(6):567-75. doi: 10.3724/sp.j.1005.2011.00567.
8
The streptomyces genome contains multiple pseudo-attB sites for the (phi)C31-encoded site-specific recombination system.链霉菌基因组包含多个用于（phi）C31编码的位点特异性重组系统的假attB位点。
J Bacteriol. 2002 Oct;184(20):5746-52. doi: 10.1128/JB.184.20.5746-5752.2002.
9
Recombination directionality factor gp3 binds ϕC31 integrase via the zinc domain, potentially affecting the trajectory of the coiled-coil motif.重组方向因子 gp3 通过锌指结构域结合 ϕC31 整合酶，可能会影响卷曲螺旋基序的轨迹。
Nucleic Acids Res. 2018 Feb 16;46(3):1308-1320. doi: 10.1093/nar/gkx1233.
10
Site-specific integration of bacteriophage VWB genome into Streptomyces venezuelae and construction of a VWB-based integrative vector.噬菌体VWB基因组在委内瑞拉链霉菌中的位点特异性整合及基于VWB的整合载体构建
Microbiology (Reading). 1998 Dec;144 ( Pt 12):3351-3358. doi: 10.1099/00221287-144-12-3351.
引用本文的文献
1
Peptidoglycan-reshuffling proteins SCO0954, SCO1758, SCO4439, and SCO4440 modulate the formation of wall-deficient cells in Streptomyces coelicolor under hyperosmotic sucrose stress.肽聚糖重排蛋白SCO0954、SCO1758、SCO4439和SCO4440在高渗蔗糖胁迫下调节天蓝色链霉菌中细胞壁缺陷型细胞的形成。
Sci Rep. 2025 Sep 1;15(1):32112. doi: 10.1038/s41598-025-15457-z.
2
Streptomyces uses both polar and dispersed cell wall synthesis during exploratory growth.链霉菌在探索性生长过程中同时利用极性和分散性细胞壁合成。
Nat Microbiol. 2025 Aug 8. doi: 10.1038/s41564-025-02080-x.
3
Streptomyces venezuelae uses secreted chitinases and a designated ABC transporter to support the competitive saprophytic catabolism of chitin.委内瑞拉链霉菌利用分泌的几丁质酶和一种特定的ABC转运蛋白来支持几丁质的竞争性腐生分解代谢。
PLoS Biol. 2025 Aug 6;23(8):e3003292. doi: 10.1371/journal.pbio.3003292. eCollection 2025 Aug.
4
Engineering the TCA cycle regulator GarA to increase erythromycin production in .改造三羧酸循环调节因子GarA以提高红霉素产量 。 （原文句末“in.”似乎不完整，推测可能是在某个特定物种中，这里按照现有内容准确翻译）
Microbiology (Reading). 2025 Aug;171(8). doi: 10.1099/mic.0.001583.
5
Role of a FAD-dependent monooxygenase in diazo group functionalization of kinamycin in .黄素腺嘌呤二核苷酸（FAD）依赖性单加氧酶在链黑菌素重氮基团功能化中的作用 。 （你提供的原文最后似乎不完整，少了具体的反应体系或环境等描述）
Microbiology (Reading). 2025 Jun;171(6). doi: 10.1099/mic.0.001576.
6
Mitigating genetic instability caused by the excision activity of the C31 integrase in .减轻由C31整合酶在……中的切除活性所引起的基因不稳定性。
Appl Environ Microbiol. 2025 Jan 31;91(1):e0181224. doi: 10.1128/aem.01812-24. Epub 2024 Dec 20.
7
Variable orthogonality of serine integrase interactions within the ϕC31 family.丝氨酸整合酶在 ϕC31 家族内的相互作用具有变向正交性。
Sci Rep. 2024 Nov 1;14(1):26280. doi: 10.1038/s41598-024-77570-9.
8
Heterologous expression of lasso peptides with apparent participation in the morphological development in Streptomyces.套索肽的异源表达明显参与链霉菌的形态发育。
AMB Express. 2024 Sep 3;14(1):97. doi: 10.1186/s13568-024-01761-w.
9
Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002.链霉菌基因间共轭辅助质粒pUZ8002的序列与起源
Access Microbiol. 2024 Jun 5;6(6). doi: 10.1099/acmi.0.000808.v3. eCollection 2024.
10
SVEN_5003 is a Major Developmental Regulator in Streptomyces venezuelae.SVEN_5003 是链霉菌属中的一个重要发育调控因子。
Curr Microbiol. 2024 May 9;81(6):166. doi: 10.1007/s00284-024-03688-8.
本文引用的文献
1
The streptomyces genome contains multiple pseudo-attB sites for the (phi)C31-encoded site-specific recombination system.链霉菌基因组包含多个用于（phi）C31编码的位点特异性重组系统的假attB位点。
J Bacteriol. 2002 Oct;184(20):5746-52. doi: 10.1128/JB.184.20.5746-5752.2002.
2
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).模式放线菌天蓝色链霉菌A3(2)的全基因组序列
Nature. 2002 May 9;417(6885):141-7. doi: 10.1038/417141a.
3
Diversity in the serine recombinases.丝氨酸重组酶的多样性。
Mol Microbiol. 2002 Apr;44(2):299-307. doi: 10.1046/j.1365-2958.2002.02891.x.
4
Resolvase-like recombination performed by the TP901-1 integrase.由TP901-1整合酶进行的类解离酶重组。
Microbiology (Reading). 2001 Aug;147(Pt 8):2051-2063. doi: 10.1099/00221287-147-8-2051.
5
Genetic manipulation of antibiotic-producing Streptomyces.产抗生素链霉菌的基因操作
Trends Microbiol. 1998 Feb;6(2):76-83. doi: 10.1016/S0966-842X(97)01161-X.
6
Hypertransposing derivatives of the streptomycete insertion sequence IS493.链霉菌插入序列IS493的超转座衍生物
Gene. 1994 Sep 15;147(1):47-54. doi: 10.1016/0378-1119(94)90037-x.
7
Transmembrane helices predicted at 95% accuracy.预测的跨膜螺旋准确率达95%。
Protein Sci. 1995 Mar;4(3):521-33. doi: 10.1002/pro.5560040318.
8
Two genes involved in the phase-variable phi C31 resistance mechanism of Streptomyces coelicolor A3(2).参与天蓝色链霉菌A3(2)相变可变phi C31抗性机制的两个基因。
J Bacteriol. 1995 Aug;177(16):4681-9. doi: 10.1128/jb.177.16.4681-4689.1995.
9
Use of vectorette and subvectorette PCR to isolate transgene flanking DNA.使用载体连接片段和亚载体连接片段PCR分离转基因侧翼DNA。
PCR Methods Appl. 1994 Oct;4(2):71-5. doi: 10.1101/gr.4.2.71.
10
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.用于将DNA从大肠杆菌接合转移至链霉菌属细菌的质粒克隆载体
Gene. 1992 Jul 1;116(1):43-9. doi: 10.1016/0378-1119(92)90627-2.
Zotero 插件