Langness U, Decius W
Arzneimittelforschung. 1978;28(12):2202-7.
L 929 mouse fibroblasts were cultured as monolayers. Proliferation and glycosaminoglycan content in cells and medium were measured, the different glycosaminoglycans fractionated. It was shown that logarithmic cell growth is followed by a stationary phase. Glycosaminoglycan synthesis started later than did cell growth and continued during stationary phase. The glycosaminoglycan pattern changed with the phase of proliferation. All mesenchyma-active substances reduced proliferation and glycosaminoglycan synthesis significantly when added in therapeutical concentrations. The fractionation of the glycosaminoglycans after addition of the exclusively suppressive substances revealed different effectivity on the different components. All substances were directly effective on the connective tissue forming cells as had been expected from clinical experience and former experiments.
将L929小鼠成纤维细胞培养成单层。测量细胞和培养基中的增殖情况以及糖胺聚糖含量，对不同的糖胺聚糖进行分级分离。结果表明，细胞对数生长之后是静止期。糖胺聚糖合成比细胞生长开始得晚，并在静止期持续进行。糖胺聚糖模式随增殖阶段而变化。当以治疗浓度添加时，所有间充质活性物质均显著降低增殖和糖胺聚糖合成。添加仅具有抑制作用的物质后对糖胺聚糖进行分级分离，结果显示对不同成分具有不同的有效性。正如临床经验和先前实验所预期的那样，所有物质对结缔组织形成细胞均具有直接作用。
