Pułaski L, Bartosz G
Department of Molecular Biophysics, University of Lódź, Poland.
Biochem Mol Biol Int. 1995 Aug;36(5):935-42.
Effect of inhibitors on and pH dependence of the export of dinitrophenyl-S-glutathione (DNP-SG), a glutathione S-conjugate formed upon in vivo conjugation of 1-chloro-2,4-dinitrobenzene to glutathione, was studied in intact human erythrocytes. The transport was inhibited by orthovanadate (IC50 = 80 microM) and fluoride (IC50 = 9 nM). Erythrocyte anion exchange (Band 3) protein inhibitors DIDS and SITS did not exert any significant effect on the transport. The transport rate increased with increasing extracellular pH in the range of 6.0-8.0. The Arrhenius activation energy of the process was 72.4 +/- 1.3 kJ/mol. Membrane potential, extracellular sodium/potassium concentration ratio and extracellular osmolality in the range 300-600 mOsm did not influence the export rate.
在完整的人红细胞中研究了抑制剂对二硝基苯基-S-谷胱甘肽（DNP-SG）转运的影响及其pH依赖性，DNP-SG是1-氯-2,4-二硝基苯在体内与谷胱甘肽结合形成的谷胱甘肽S-共轭物。该转运受到原钒酸盐（IC50 = 80 microM）和氟化物（IC50 = 9 nM）的抑制。红细胞阴离子交换（带3）蛋白抑制剂DIDS和SITS对转运没有显著影响。在6.0-8.0范围内，转运速率随细胞外pH升高而增加。该过程的阿仑尼乌斯活化能为72.4±1.3 kJ/mol。膜电位、细胞外钠/钾浓度比以及300-600 mOsm范围内的细胞外渗透压均不影响转运速率。
