Soszyński M, Skalski Z, Pułaski L, Bartosz G
Department of Molecular Biophysics, University of Lódź, Poland.
Biochem Mol Biol Int. 1995 Oct;37(3):537-45.
Tert-Butyl hydroperoxide (100-300 microM) was found to inhibit the active efflux of dinitrophenyl-S-glutathione (DNP-SG) from human erythrocytes. From among amino acid peroxides generated by irradiation of amino acid (proline, valine and leucine) solutions, valine hydroperoxide (150 microM) had a similar effect on the DNP-SG transport. As the transport of glutathione S-conjugates is an important step of cellular detoxication, these results indicate that oxidative stress may impair cellular resistance to chemical stress of other kinds. t-Butyl and amino acid peroxides upon interaction with hemoglobin and whole erythrocytes produce free radicals which may be responsible for the damage to the glutathione S-conjugate pump of the erythrocyte membrane.
已发现叔丁基过氧化氢（100 - 300微摩尔）可抑制二硝基苯基 - S - 谷胱甘肽（DNP - SG）从人红细胞中的主动外排。在通过照射氨基酸（脯氨酸、缬氨酸和亮氨酸）溶液产生的氨基酸过氧化物中，缬氨酸过氧化氢（150微摩尔）对DNP - SG转运有类似作用。由于谷胱甘肽S - 共轭物的转运是细胞解毒的重要步骤，这些结果表明氧化应激可能损害细胞对其他种类化学应激的抵抗力。叔丁基和氨基酸过氧化物与血红蛋白和全红细胞相互作用时会产生自由基，这可能是红细胞膜谷胱甘肽S - 共轭物泵受损的原因。
