Rees W D, Rhodes J, Williams B C, Owen E, Newcombe R G
Gastroenterology. 1978 Mar;74(3):492-5.
The source of the histamine released during damage to the gastric mucosa has been investigated in rats using perfused total gastric pouches. Two groups of rats were treated with either intraperitoneal normal saline or compound 48/80, and agent that produces mast cell degranulation, over a 5-day period. On the 5th day, total gastric pouches were prepared and connected to a perfusion circuit that enabled a 20-ml volume to be circulated through the pouches. The experiments consisted of three 30-min periods during which transmucosal potential difference was monitored and ionic (hydrogen and sodium) flux measured; standard acid solution was used in the first two periods and a taurocholate solution in the third. Sodium taurocholate produced a significant increase in ionic flux and fall in the potential difference, the magnitude of the changes being similar in the 48/80- and saline-treated groups. Histamine was released from the mucosa in significantly greater amounts during the taurocholate period, and the increase was similar in both groups of rats. Histological examination of the stomachs confirmed mast cell degranulation in the 48/80-treated groups. We conclude that the histamine released during mucosal damage is probably derived from the "nonmast cell pool" and that this histamine may play a role in mediating the mucosal damage.
利用灌注全胃囊袋法在大鼠中研究了胃黏膜损伤时释放的组胺来源。两组大鼠在5天时间内分别接受腹腔注射生理盐水或化合物48/80（一种可使肥大细胞脱颗粒的药物）治疗。在第5天，制备全胃囊袋并连接到一个灌注回路，使20毫升的液体在囊袋中循环。实验包括三个30分钟的时间段，在此期间监测跨黏膜电位差并测量离子（氢离子和钠离子）通量；在前两个时间段使用标准酸溶液，在第三个时间段使用牛磺胆酸盐溶液。牛磺胆酸钠使离子通量显著增加，电位差下降，48/80处理组和生理盐水处理组的变化幅度相似。在牛磺胆酸盐处理期间，组胺从黏膜中释放的量显著增加，两组大鼠的增加情况相似。对胃的组织学检查证实48/80处理组存在肥大细胞脱颗粒。我们得出结论，黏膜损伤时释放的组胺可能来源于“非肥大细胞池”，并且这种组胺可能在介导黏膜损伤中起作用。
