Proulx M, Maupoil V, Rochette L, du Souich P
Département de pharmacologie, Faculté de Médecine, Université de Montréal, Québec, Canada.
Free Radic Res. 1995 Sep;23(3):245-54. doi: 10.3109/10715769509064037.
In the rabbit, an acute inflammatory reaction triggered by the subcutaneous administration of turpentine induces in hepatic tissues an oxidative stress, as well as a decrease in activity of enzymatic scavengers of reactive oxygen species (ROS). The objective of this study was to investigate, the repercussions of a local inflammatory reaction on the antioxidant capacity and markers of systemic oxidative stress in plasma. To this purpose, rabbits received a.s.c. injection of turpentine (5 mL/kg) or NaCl 0.9% (w/v). Blood samples were collected at different times during the 48 hours of the experiment to evaluate: firstly, the antilipoperoxidant activity of plasma by measuring the inhibition of autoxidation of brain homogenate, and the concentrations of tocopherol and ascorbic acid; secondly, the severity of oxidative stress in plasma by assaying the concentration of thiobarbituric acid reactive substances (TBARS), and the concentration of ascorbyl radical. The results show that the antilipoperoxidant capacity of plasma gradually increased to be 167% higher than baseline values (p < 0.05) after 48 hours of experiment. alpha-Tocopherol and ascorbic acid levels increased by 49% and 80%, respectively (p < 0.05) during the first 24 hours. Lipid peroxidation continuously increased to be 98% higher than baseline values (p < 0.05) at 48 hours, while ascorbyl radical levels were not modified (p < 0.05). In summary, an acute local inflammatory reaction causes a steady progression of oxidative stress, while it stimulates the antilipoperoxidant activity of plasma, to which alpha-tocopherol and ascorbic acid appear to contribute, essentially early in the inflammation.
在家兔中，皮下注射松节油引发的急性炎症反应会在肝组织中诱导氧化应激，同时导致活性氧（ROS）酶清除剂的活性降低。本研究的目的是调查局部炎症反应对血浆抗氧化能力和全身氧化应激标志物的影响。为此，给家兔皮下注射松节油（5 mL/kg）或0.9%（w/v）的氯化钠溶液。在实验的48小时内不同时间采集血样，以评估：首先，通过测量脑匀浆自氧化的抑制情况、生育酚和抗坏血酸的浓度来评估血浆的抗脂质过氧化活性；其次，通过检测硫代巴比妥酸反应性物质（TBARS）的浓度和抗坏血酸自由基的浓度来评估血浆氧化应激的严重程度。结果表明，实验48小时后，血浆的抗脂质过氧化能力逐渐增加，比基线值高167%（p < 0.05）。在前24小时内，α-生育酚和抗坏血酸水平分别增加了49%和80%（p < 0.05）。脂质过氧化在48小时时持续增加，比基线值高98%（p < 0.05），而抗坏血酸自由基水平未发生改变（p < 0.05）。总之，急性局部炎症反应会导致氧化应激稳步进展，同时刺激血浆的抗脂质过氧化活性，α-生育酚和抗坏血酸似乎在炎症早期对此起主要作用。
