Zarzycki P K, Kowalski P, Nowakowska J, Lamparczyk H
Medical Academy, Faculty of Pharmacy, Gdańsk, Poland.
J Chromatogr A. 1995 Aug 11;709(1):203-8. doi: 10.1016/0021-9673(95)00247-k.
Two methods are described based on high-performance liquid chromatography and capillary electrophoresis that provide the selective and sensitive determination of nicotinic acid in human plasma. Moreover, the capillary electrophoresis system was used for the separation of nicotinic acid, nicotinamide, nicotinamide N-oxide, N'-methylnicotinamide, 6-hydroxynicontinic acid, nicotinuric acid and barbital (internal standard). The extraction procedure is simple; no gradient elution or derivatization is required. Both methods can be useful for clinical and biomedical investigations.
本文描述了两种基于高效液相色谱和毛细管电泳的方法，可用于选择性、灵敏地测定人血浆中的烟酸。此外，毛细管电泳系统用于分离烟酸、烟酰胺、烟酰胺氮氧化物、N'-甲基烟酰胺、6-羟基烟酸、烟尿酸和巴比妥（内标）。提取过程简单；无需梯度洗脱或衍生化。这两种方法都可用于临床和生物医学研究。
