Shihabi Z K
Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
J Chromatogr B Biomed Appl. 1995 Jul 7;669(1):53-8. doi: 10.1016/0378-4347(95)00052-k.
Capillary electrophoresis was used in this study to separate urinary myoglobin from hemoglobin based on its electrophoretic mobility. Urine was applied directly without any treatment. The separation was accomplished in less than 7 min. Myoglobin extracted from human muscle tissues was separated, in a borate buffer 150 mM, pH 8.7 containing 0.5% polyethyleneglycol at 6 kV, into two peaks (MI and MII) which were also resolved far from hemoglobin. Upon standing at room temperature, MII converted into MI. Horse myoglobin eluted close to MI. The addition of polyethyleneglycol to the buffer enhanced the separation and increased the peak height of myoglobin. Optimum conditions for the separation are discussed. The method is suitable for routine clinical analysis because of its simplicity and speed.
本研究采用毛细管电泳法，根据尿肌红蛋白和血红蛋白的电泳迁移率来分离它们。尿液未经任何处理直接进样。分离在不到7分钟内完成。从人肌肉组织中提取的肌红蛋白，在含有0.5%聚乙二醇的150 mM、pH 8.7的硼酸盐缓冲液中，于6 kV电压下分离为两个峰（MI和MII），这两个峰也与血红蛋白分离得很远。室温放置时，MII转化为MI。马肌红蛋白的洗脱位置接近MI。向缓冲液中添加聚乙二醇可增强分离效果并增加肌红蛋白的峰高。文中讨论了分离的最佳条件。该方法因其简单性和快速性而适用于常规临床分析。
