Brennand J E, Leask R, Kelly R W, Greer I A, Calder A A
Department of Obstetrics and Gynaecology, University of Glasgow, UK.
Eur J Endocrinol. 1995 Nov;133(5):527-33. doi: 10.1530/eje.0.1330527.
The objective was to compare the changes in prostaglandin synthesis and metabolism occurring within the fetal membranes that are associated with the onset of parturition and to study the effect of steroid hormones on prostaglandin metabolism. A tissue explant study was made of discs of amnion and chorion obtained from 24 pregnant women at 37-42 weeks' gestation following spontaneous labour and delivery (12 women) and elective caesarean section (12 women). Significantly more prostaglandin E2 (PGE2) and PGF2 alpha were synthesized by amnion obtained following spontaneous labour than elective caesarean section. Arachidonic acid stimulated both PGE2 and PGF2 alpha synthesis by amnion in both groups. Phorbol myristoyl acetate stimulated PGE2 synthesis in both groups. There was no difference between the groups in the capacity of the chorion to metabolize prostaglandins. Mifepristone (RU 486) reduced the metabolism of added PGE2 following spontaneous labour, while dexamethasone and progesterone had no effect on prostaglandin metabolism. In conclusion, the increase in concentration of PGE2 and PGF2 alpha associated with the onset of spontaneous labour is the result of an increase in synthesis rather than a reduction in metabolism. There was no decrease in metabolism to account for the increase in prostaglandin concentrations and, with the exception of mifepristone, metabolism was not altered by the addition of steroid hormones.
目的是比较与分娩发动相关的胎膜内前列腺素合成与代谢的变化，并研究甾体激素对前列腺素代谢的影响。对从24名妊娠37 - 42周的孕妇获取的羊膜和绒毛膜圆盘进行组织外植体研究，这些孕妇分别经历了自然分娩和分娩（12名妇女）以及选择性剖宫产（12名妇女）。自然分娩后获得的羊膜合成的前列腺素E2（PGE2）和前列腺素F2α明显多于选择性剖宫产。花生四烯酸刺激两组羊膜中PGE2和PGF2α的合成。佛波醇肉豆蔻酸酯刺激两组中PGE2的合成。绒毛膜代谢前列腺素的能力在两组之间没有差异。米非司酮（RU 486）减少了自然分娩后添加的PGE2的代谢，而地塞米松和孕酮对前列腺素代谢没有影响。总之，与自然分娩发动相关的PGE2和PGF2α浓度增加是合成增加而非代谢减少的结果。没有代谢减少来解释前列腺素浓度的增加，并且除米非司酮外，添加甾体激素不会改变代谢。
