Hong Y, Wong T, Bourke B, Chan V L
Department of Microbiology, University of Toronto, Ontario, Canada.
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2561-7. doi: 10.1099/13500872-141-10-2561.
A complete isoleucyl-tRNA synthetase gene (ileS) of Campylobacter jejuni was isolated from a C. jejuni TGH9011 genomic DNA library constructed in pBluescript. The complete coding sequence, flanking regions and transcription start point were determined. The deduced isoleucyl-tRNA synthetase (IleRS) had 917 amino acids with a molecular mass of 105,399 Da, which was consistent with the observed size of 105 kDa in Escherichia coli maxicells. The ileS gene was mapped onto the physical map of the C. jejuni genome. Alignment of the C. jejuni IleRS sequence with six other bacterial IleRS sequences and two lower eukaryotic IleRS sequences identified seven conserved motifs, including the two signature sequences, HIGH and KMSKS, of class I aminoacyl-tRNA synthetases.
从构建于pBluescript的空肠弯曲菌TGH9011基因组DNA文库中分离出空肠弯曲菌完整的异亮氨酰 - tRNA合成酶基因（ileS）。确定了完整的编码序列、侧翼区域和转录起始点。推导的异亮氨酰 - tRNA合成酶（IleRS）有917个氨基酸，分子量为105,399 Da，这与在大肠杆菌大细胞中观察到的105 kDa大小一致。ileS基因被定位到空肠弯曲菌基因组的物理图谱上。将空肠弯曲菌IleRS序列与其他六种细菌IleRS序列和两种低等真核生物IleRS序列进行比对，鉴定出七个保守基序，包括I类氨酰 - tRNA合成酶的两个特征序列HIGH和KMSKS。
