Chan V L, Bingham H L
Department of Microbiology, University of Toronto, Ontario, Canada.
J Bacteriol. 1992 Feb;174(3):695-701. doi: 10.1128/jb.174.3.695-701.1992.
We report the cloning and complete nucleotide sequence of the Campylobacter jejuni lysyl-tRNA synthetase gene (lysS). The C. jejuni lysS gene sequence shows high homology to the two Escherichia coli lysyl-tRNA synthetase genes, lysS and lysU. The Campylobacter lysyl-tRNA synthetase protein (LysRS) shows 47.9 and 46.6% sequence identity to the E. coli enzymes encoded by the lysS and lysU genes, respectively. The LysRS encoded by the C. jejuni gene is a polypeptide of 501 amino acids with a deduced molecular weight of 57,867. The enzyme is active in E. coli. The gene is expressed from its own promoter, and the transcription start site has been mapped. The carboxyl-terminal codon of the C. jejuni lysS gene overlaps by 1 bp with the Met initiation codon of the glyA gene, which has been shown to have a promoter which is functional in E. coli (V.L. Chan and H.L. Bingham, Gene 101:51-58, 1991). C. jejuni, unlike E. coli, has only one lysyl-tRNA synthetase gene.
我们报道了空肠弯曲菌赖氨酰 - tRNA合成酶基因（lysS）的克隆及完整核苷酸序列。空肠弯曲菌lysS基因序列与两个大肠杆菌赖氨酰 - tRNA合成酶基因lysS和lysU具有高度同源性。空肠弯曲菌赖氨酰 - tRNA合成酶蛋白（LysRS）与lysS和lysU基因编码的大肠杆菌酶的序列同一性分别为47.9%和46.6%。空肠弯曲菌基因编码的LysRS是一个由501个氨基酸组成的多肽，推导分子量为57,867。该酶在大肠杆菌中具有活性。该基因由其自身启动子表达，转录起始位点已被定位。空肠弯曲菌lysS基因的羧基末端密码子与glyA基因的Met起始密码子重叠1个碱基对，已证明glyA基因具有在大肠杆菌中起作用的启动子（V.L. Chan和H.L. Bingham，《基因》101:51 - 58，1991）。与大肠杆菌不同，空肠弯曲菌只有一个赖氨酰 - tRNA合成酶基因。
