一种编码尼龙低聚物降解酶的质粒：pOAD2的核苷酸序列及分析

A plasmid encoding enzymes for nylon oligomer degradation: nucleotide sequence and analysis of pOAD2.

作者信息

Kato K, Ohtsuki K, Koda Y, Maekawa T, Yomo T, Negoro S, Urabe I

机构信息

Department of Biotechnology, Osaka University, Japan.

出版信息

Microbiology (Reading). 1995 Oct;141 ( Pt 10):2585-90. doi: 10.1099/13500872-141-10-2585.

DOI:10.1099/13500872-141-10-2585

PMID:7582019

Abstract

The entire nucleotide sequence of nylon oligomer degradative plasmid pOAD2 from Flavobacterium sp. KI723T1 was determined. pOAD2 comprises 45519 bp, with a 66.6 mol% G+C content. The precise loci of the four nylon oligomer degradation genes, namely nylA (6-aminohexanoate-cyclic-dimer hydrolase gene), nylB (6-aminohexanoate-dimer hydrolase), nylB' (a gene having 88% homology to nylB) and nylC (endo-type 6-aminohexanoate oligomer hydrolase), and five IS6100 elements were identified on this plasmid. Comparison of the sequence of pOAD2 with those in the GenBank and EMBL databases revealed that the deduced amino acid sequences from eight regions of pOAD2 had significant similarity with the sequences of gene products such as oppA-F (oligopeptide permeases), ftsX (filamentation temperature sensitive), penDE (isopenicillin N-acyltransferase) and rep (plasmid incompatibility). A functional map of pOAD2 is presented.

摘要

测定了来自黄杆菌属KI723T1的尼龙寡聚物降解质粒pOAD2的完整核苷酸序列。pOAD2包含45519 bp，G+C含量为66.6 mol%。在该质粒上鉴定出了四个尼龙寡聚物降解基因，即nylA（6-氨基己酸环二聚体水解酶基因）、nylB（6-氨基己酸二聚体水解酶）、nylB'（与nylB具有88%同源性的基因）和nylC（内切型6-氨基己酸寡聚物水解酶）的精确位点，以及五个IS6100元件。将pOAD2的序列与GenBank和EMBL数据库中的序列进行比较，结果显示pOAD2八个区域推导的氨基酸序列与oppA-F（寡肽通透酶）、ftsX（丝状温度敏感蛋白）、penDE（异青霉素N-酰基转移酶）和rep（质粒不相容性）等基因产物的序列具有显著相似性。给出了pOAD2的功能图谱。