来自嗜碱细菌土壤杆菌属菌株KY5R和考克氏菌属菌株KY2的6-氨基己酸酯寡聚体水解酶
6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.
作者信息
Yasuhira Kengo, Tanaka Yasuhito, Shibata Hiroshi, Kawashima Yasuyuki, Ohara Akira, Kato Dai-ichiro, Takeo Masahiro, Negoro Seiji
机构信息
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
出版信息
Appl Environ Microbiol. 2007 Nov;73(21):7099-102. doi: 10.1128/AEM.00777-07. Epub 2007 Sep 7.
Abstract
Alkalophilic, nylon oligomer-degrading strains, Agromyces sp. and Kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant. The 6-aminohexanoate oligomer hydrolases (NylC) from the alkalophilic strains had 95.8 to 98.6% similarity to the enzyme in neutrophilic Arthrobacter sp. but had superior thermostability, activity under alkaline conditions, and affinity for nylon-related substrates, which would be advantageous for biotechnological applications.
摘要
嗜碱的、能降解尼龙低聚物的菌株，即土壤杆菌属（Agromyces sp.）和考克氏菌属（Kocuria sp.），是从一家尼龙6工厂的废水以及污水处理厂的活性污泥中分离出来的。来自嗜碱菌株的6-氨基己酸低聚物水解酶（NylC）与嗜中性节杆菌属（Arthrobacter sp.）中的酶具有95.8%至98.6%的相似性，但具有更高的热稳定性、在碱性条件下的活性以及对尼龙相关底物的亲和力，这对于生物技术应用将是有利的。
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