Jin Y, Tipoe G L, White F H, Yang L
Department of Anatomy, Faculty of Medicine, University of Hong Kong.
Virchows Arch. 1995;427(2):145-51. doi: 10.1007/BF00196519.
The present study was designed to determine whether increased vascularity occurs during malignant transformation of human oral cheek epithelium. Nine normal (N) samples were taken from the resection margins of benign lesions; the pathological lesions were classified as chronic inflammation (CI; n = 11), fibrous hyperplasia (FH; n = 12), lichen planus (LIP; n = 8), dysplasia (DYS; n = 5), squamous cell carcinoma (SCC; n = 25; well differentiated [SCCWD]; n = 10; moderately to poorly differentiated [SCCMPD]; n = 15) and epithelium adjacent to carcinomas (EAC; n = 6). Sections were stained with monoclonal antibody (mAb) against vimentin using an ABC immunoperoxidase technique. All blood vessels present within a depth of 0.9 mm of lamina propria were quantified irrespective of their morphology. The blood vessel parameters quantified were volume density (VVBV, CT), number per unit area (NABV, CT), length per unit volume (LVBV, CT) and mean transverse sectional area (ABV). VVBV, CT increased significantly between normal and all pathological groups. Amongst the pathological groups, statistical differences were detected between CI and SCC, CI and EAC, FH and SCCWD, FH and EAC, LIP and SCC, LIP and EAC, DYS and SCCWD and DYS and EAC. The EAC group had the highest VVBV, CT and the values of NABV, CT and LVBV, CT were significantly higher in all the pathological groups when compared with the normal group. No significant differences were detected between any of the pathological group. The parameter ABV increased significantly between normal and DYS, FH, SCC, EAC, FH and EAC, FH and SCC, CI and EAC, CI and SCC, LIP and EAC and LIP and SCC. Spearman rank correlations detected a positive correlation between the severity of oral lesions and all of the blood vessel parameters. We conclude that a mAb against vimentin improved the identification of smaller blood vessels and the blood vessel data suggest that angiogenesis occurs in premalignant and malignant lesions of human oral cheek epithelium. Angiogenesis seems to play an essential role in sustaining the actively growing and transforming cells.
本研究旨在确定人类口腔颊黏膜上皮恶性转化过程中血管生成是否增加。从良性病变切除边缘获取9个正常（N）样本；病理病变分类为慢性炎症（CI；n = 11）、纤维增生（FH；n = 12）、扁平苔藓（LIP；n = 8）、发育异常（DYS；n = 5）、鳞状细胞癌（SCC；n = 25；高分化[SCCWD]；n = 10；中低分化[SCCMPD]；n = 15）以及癌旁上皮（EAC；n = 6）。切片使用ABC免疫过氧化物酶技术用抗波形蛋白单克隆抗体（mAb）染色。对固有层0.9mm深度内的所有血管进行定量，无论其形态如何。定量的血管参数为体积密度（VVBV，CT）、单位面积数量（NABV，CT）、单位体积长度（LVBV，CT）和平均横截面积（ABV）。正常组与所有病理组之间VVBV，CT显著增加。在病理组中，检测到CI与SCC、CI与EAC、FH与SCCWD、FH与EAC、LIP与SCC、LIP与EAC、DYS与SCCWD以及DYS与EAC之间存在统计学差异。EAC组的VVBV，CT最高，与正常组相比，所有病理组的NABV，CT和LVBV，CT值均显著更高。在任何病理组之间均未检测到显著差异。正常组与DYS、FH、SCC、EAC、FH与EAC、FH与SCC、CI与EAC、CI与SCC、LIP与EAC以及LIP与SCC之间ABV参数显著增加。Spearman等级相关性检测到口腔病变严重程度与所有血管参数之间呈正相关。我们得出结论，抗波形蛋白单克隆抗体改善了对较小血管的识别，并且血管数据表明血管生成发生在人类口腔颊黏膜上皮的癌前病变和恶性病变中。血管生成似乎在维持活跃生长和转化的细胞中起重要作用。
