Khan Yusra, Rehani Shweta, Sharma Mohit
Department of Oral Pathology, Faculty of Dentistry, Jamia Millia Islamia, New Delhi, India.
Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India.
J Oral Maxillofac Pathol. 2024 Jul-Sep;28(3):403-408. doi: 10.4103/jomfp.jomfp_17_24. Epub 2024 Oct 15.
Oral cancer is the sixth most common cancer, and 90% of them are oral squamous cell carcinomas (OSCC). As most OSCC are asymptomatic and are only detected at an advanced stage, the 5-year survival rate is only 50%. Thus, using novel prognosticators can minimise mortality and morbidity associated with OSCC. This study aims to evaluate the relationship between mast cells and angiogenesis in different grades of OSCC to analyse their role in its progression.
A total of 45 cases were included, comprising 10 well-differentiated SCCs (WDOSCC), 10 moderately differentiated SCCs (MDOSCC), and 10 poorly differentiated SCCs (PDOSCC). Additionally, five normal buccal mucosae (NBM) samples served as negative controls for OSCC. Five cases of neurofibroma and pyogenic granuloma were used as positive controls for mast cells and angiogenesis, respectively.
The mean MCD in WDOSCC, MDOSCC, and PDOSCC were 3.2620 ± 2.65177, 3.0310 ± 1.38276, and 4.1580 ± 2.49482, respectively. The MVD in WDOSCC, MDOSCC, and PDOSCC were 10.2850 ± 4.35032, 9.9240 ± 2.72533, and 7.1520 ± 2.26966, respectively.
MCD was the highest in PDOSCC, followed by WDOSCC and MDOSCC. These results indicate a redundant role of mast cells in OSCC, or they might jumpstart malignancy but are retarded with OSCC progression. The MVD decreased with higher grades, in contrast to the prevalent literature. The correlation analysis between MVD and MCD revealed no significant correlation between them.
We found a non-significant role of mast cells in tumour biology and a decrease in vascularity with advancing grades. These results indicate a lower need for mast cell activation to augment vascularisation. A study with a larger sample size is needed to confirm our results.
口腔癌是第六大常见癌症，其中90%为口腔鳞状细胞癌（OSCC）。由于大多数OSCC无症状，仅在晚期才被发现，其5年生存率仅为50%。因此，使用新型预后指标可将与OSCC相关的死亡率和发病率降至最低。本研究旨在评估不同分级的OSCC中肥大细胞与血管生成之间的关系，以分析它们在其进展中的作用。
共纳入45例病例，包括10例高分化鳞状细胞癌（WDOSCC）、10例中分化鳞状细胞癌（MDOSCC）和10例低分化鳞状细胞癌（PDOSCC）。此外，5例正常颊黏膜（NBM）样本作为OSCC的阴性对照。5例神经纤维瘤和化脓性肉芽肿分别作为肥大细胞和血管生成的阳性对照。
WDOSCC、MDOSCC和PDOSCC的平均肥大细胞计数（MCD）分别为3.2620±2.65177、3.0310±1.38276和4.1580±2.49482。WDOSCC、MDOSCC和PDOSCC的微血管密度（MVD）分别为10.2850±4.35032、9.9240±2.72533和7.1520±2.26966。
PDOSCC中的MCD最高，其次是WDOSCC和MDOSCC。这些结果表明肥大细胞在OSCC中具有多余的作用，或者它们可能启动恶性肿瘤，但随着OSCC进展而受到抑制。与现有文献相反，MVD随着分级升高而降低。MVD与MCD之间的相关性分析显示它们之间无显著相关性。
我们发现肥大细胞在肿瘤生物学中作用不显著，且随着分级升高血管化程度降低。这些结果表明增强血管化对肥大细胞激活的需求较低。需要进行更大样本量的研究来证实我们的结果。
