de Angelis L, Furlan C
Institute of Pharmacology, University of Trieste, Italy.
Neurobiol Learn Mem. 1995 Sep;64(2):119-24. doi: 10.1006/nlme.1995.1050.
The effects of a nootropic drug, oxiracetam (50-100-200 mg/kg ip), and a potent antioxidant agent, ascorbic acid (62.5-125-250 mg/kg ip), administered alone or in combination, were investigated on scopolamine-induced amnesia in a mouse habituation test. The light-dark aversion test was selected and was carried out in aged mice. Habituation to the test box occurred over a 3-day period, control mice showing a significant between-day increase in the time spent in the dark box, but not in the number of transitions. On Day 4, following post-trial administration over a 3-day period of oxiracetam (50-100 and 200 mg/kg ip) or ascorbic acid (62.5-125 and 250 mg/kg ip), a significant between-day increase in the time spent in the black area, but not in the number of transitions, was found. The combination of oxiracetam (100 mg/kg ip) with ascorbic acid (125 mg/kg ip) produced a similar pattern of results. The acute administration of scopolamine (0.25 mg/kg ip) to mice treated over a 3-day period with vehicle disrupted the habituation response. In mice that had received the 3-day treatment with oxiracetam or ascorbic acid or its combination, scopolamine failed to alter significantly the learning pattern. In conclusion, these data demonstrate that ascorbic acid, alone or in combination with oxiracetam, may prevent experimentally induced amnesia in aged mice.
在小鼠习惯化试验中，研究了促智药奥拉西坦（腹腔注射50 - 100 - 200毫克/千克）和强效抗氧化剂抗坏血酸（腹腔注射62.5 - 125 - 250毫克/千克）单独或联合给药对东莨菪碱诱导的失忆的影响。选用明暗回避试验并在老年小鼠中进行。对试验箱的习惯化在3天内发生，对照小鼠在暗箱中停留的时间在日间有显著增加，但转换次数没有增加。在第4天，在对小鼠进行为期3天的奥拉西坦（腹腔注射50 - 100和200毫克/千克）或抗坏血酸（腹腔注射62.5 - 125和250毫克/千克）试验性给药后，发现小鼠在黑色区域停留的时间在日间有显著增加，但转换次数没有增加。奥拉西坦（腹腔注射100毫克/千克）与抗坏血酸（腹腔注射125毫克/千克）联合给药产生了类似的结果模式。对用赋形剂处理3天的小鼠急性腹腔注射东莨菪碱（0.25毫克/千克）破坏了习惯化反应。在接受了为期3天的奥拉西坦或抗坏血酸或其组合处理的小鼠中，东莨菪碱未能显著改变学习模式。总之，这些数据表明，抗坏血酸单独或与奥拉西坦联合使用可能预防老年小鼠实验性诱导的失忆。
