el Kafi B, Leger L, Seguin S, Jouvet M, Cespuglio R
Department of Experimental Medicine, INSERM U 52, CNRS-URA 1195, Claude Bernard University, Lyon, France.
Brain Res. 1995 Jul 24;686(2):150-9. doi: 10.1016/0006-8993(95)00390-c.
Two peptides known for their hypnogenic properties, CLIP (corticotropin-like intermediate lobe peptide or ACTH 18-39) or VIP (vasoactive intestinal polypeptide), were injected locally into the nucleus raphe dorsalis (nRD) of rats pretreated with p-chlorophenylalanine (PCPA). During the dark period, the PCPA insomnia was primarily associated with a reduction in paradoxical sleep (PS), whereas both slow wave sleep (SWS) and PS were decreased during the light period. Immunohistochemistry of serotonin in PCPA-pretreated animals indicated a clear disappearance of 5-HT fibers in the basal hypothalamus and the nRD as compared to control animals. Local injections of CLIP or VIP in the nRD restored PS and SWS. The positive injection sites corresponded to the anatomical distribution of either CLIP or VIP fibers, i.e., the entire nRD for VIP and the antero-dorsal part of this nucleus for CLIP. The sleep effects obtained in PCPA-pretreated rats involve a non-5-HT sleep permissive component within the nRD upon which these injected peptides act.
两种因其催眠特性而闻名的肽，促肾上腺皮质激素样中叶肽（CLIP，即促肾上腺皮质激素18 - 39）或血管活性肠肽（VIP），被局部注射到用对氯苯丙氨酸（PCPA）预处理的大鼠的中缝背核（nRD）中。在黑暗期，PCPA诱导的失眠主要与异相睡眠（PS）减少有关，而在光照期，慢波睡眠（SWS）和PS均减少。与对照动物相比，PCPA预处理动物中5-羟色胺的免疫组织化学显示，下丘脑基部和nRD中5-羟色胺纤维明显消失。在nRD中局部注射CLIP或VIP可恢复PS和SWS。阳性注射部位与CLIP或VIP纤维的解剖分布相对应，即VIP对应整个nRD，CLIP对应该核的前背部分。在PCPA预处理大鼠中获得的睡眠效应涉及nRD内一种非5-羟色胺的睡眠允许成分，这些注射的肽作用于该成分。
