Bayer S A
J Comp Neurol. 1979 Jan 1;183(1):89-106. doi: 10.1002/cne.901830108.
Neurogenesis in the rat septal region was examined with 3H-thymidine autoradiography. The rats in the prenatal groups were the offspring of pregnant females given two injections of 3H-thymidine on consecutive days in an overlapping series: embryonic day (E) 13 + E14, E14 + E15,. . . E21 + E22. The rats in the postnatal groups were injected in a nonoverlapping series: the day of birth and postnatal day (P) 1, P2 + P3, P3 + P4. On 60 days of age, the percentage of labelled cells and the proportion of cells added during each day of formation were determined at several anatomical levels within the midline nuclear group (nucleus of the diagonal band, medial and triangular septal nuclei), the lateral septal nucleus, and the ventrolateral nuclear group (nucleus accumbens, bed nuclei of the stria terminalis and the anterior commissure). The neurons within each nuclear group form in significantly different waves, those of the midline group forming between E13-E17, the lateral septal nucleus between E15-E19, the bed nuclei of the stria terminalis and anterior commissure between E14-E18, the nucleus accumbens between E17-P2. All nuclei and nuclear groups show characteristic gradients of formation. Both the midline nuclear group and the bed nucleus of the stria terminalis (including the commissural bed nucleus) have their earliest forming neurons lying near the crossing of the anterior commissure; younger nuerons are located both rostrally and caudally with the youngest neurons lying in the most rostral extension of the diagonal band nucleus and the strial bed nucleus. The lateral septal nucleus forms along a strong mediolateral gradient throughout its length after neurogenesis is almost complete in the midline nuclear group. Throughout the length of the nucleus accumbens, the oldest neurons are located ventrally while progressively younger cells are found dorsally beneath the inferior horn of the lateral ventricle.
采用³H-胸腺嘧啶核苷放射自显影术对大鼠隔区的神经发生进行了研究。产前组的大鼠是怀孕母鼠的后代，母鼠在连续几天内分两次注射³H-胸腺嘧啶核苷，形成重叠序列：胚胎期（E）第13天 + 第14天、第14天 + 第15天、……、第21天 + 第22天。产后组的大鼠则进行非重叠序列注射：出生当天和出生后第（P）1天、第2天 + 第3天、第3天 + 第4天。在60日龄时，在中线核群（斜角带核、内侧和三角隔核）、外侧隔核以及腹外侧核群（伏隔核、终纹床核和前连合）内的几个解剖层面，确定标记细胞的百分比以及在每个形成日添加的细胞比例。每个核群内的神经元形成明显不同的波峰，中线核群的神经元在E13 - E17之间形成，外侧隔核在E15 - E19之间形成，终纹床核和前连合在E14 - E18之间形成，伏隔核在E17 - P2之间形成。所有核和核群均显示出特征性的形成梯度。中线核群和终纹床核（包括连合床核）最早形成的神经元位于前连合交叉附近；较年轻的神经元位于吻侧和尾侧，最年轻的神经元位于斜角带核和终纹床核的最吻侧延伸处。在中线核群神经发生几乎完成后，外侧隔核沿其全长形成强烈的内外侧梯度。在伏隔核的全长范围内，最老的神经元位于腹侧，而在背侧、外侧脑室下角下方可发现逐渐年轻的细胞。
