Miyazaki A, Sakuma S, Morikawa W, Takiue T, Miake F, Terano T, Sakai M, Hakamata H, Sakamoto Y, Natio M
Department of Biochemistry, Kumamoto University School of Medicine, Japan.
Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):1882-8. doi: 10.1161/01.atv.15.11.1882.
The effects of intravenous injection of purified rabbit apoA-I on the progression of aortic atherosclerosis in cholesterol-fed rabbits were examined. In experiment 1, 28 rabbits were equally divided into groups A and B and fed a 0.5% cholesterol diet for 90 days. For the last 30 days, group B received 40 mg apoA-I every week. The fatty streak lesions in group B (23.9 +/- 15.6%) were significantly suppressed compared with those in group A (46.0 +/- 24.9%) (P < .05). In experiment 2, 33 rabbits were divided into four groups (8 or 9 rabbits per group) and fed a 0.5% cholesterol diet. Group A was killed on day 105, while groups B, C, and D were maintained for an additional 60 days on a normal diet, during which time groups C and D received 1 mg apoA-I every other day or 40 mg apoA-I every week, respectively. The lesions in group C (70.2 +/- 15.4%) and group D (65.7 +/- 20.0%) were significantly suppressed compared with those in group B (86.2 +/- 13.7%) (P < .05) but were not reduced to the level of group A (50.0 +/- 22.9%). Although apparent regression was not observed under these conditions, the present study provided the first evidence for the antiatherogenic effect of homologous and apoA-I on the progression of atherosclerosis in cholesterol-fed rabbits.
研究了静脉注射纯化的兔载脂蛋白A-I对高胆固醇喂养兔主动脉粥样硬化进展的影响。在实验1中，28只兔被平均分为A组和B组，给予0.5%胆固醇饮食90天。在最后30天，B组每周接受40mg载脂蛋白A-I。与A组（46.0±24.9%）相比，B组的脂纹病变（23.9±15.6%）受到显著抑制（P<0.05）。在实验2中，33只兔被分为四组（每组8或9只兔），给予0.5%胆固醇饮食。A组在第105天处死，而B、C和D组在正常饮食下再维持60天，在此期间，C组和D组分别每隔一天接受1mg载脂蛋白A-I或每周接受40mg载脂蛋白A-I。与B组（86.2±13.7%）相比，C组（70.2±15.4%）和D组（65.7±20.0%）的病变受到显著抑制（P<0.05），但未降至A组（50.0±22.9%）的水平。尽管在这些条件下未观察到明显的消退，但本研究为同源载脂蛋白A-I对高胆固醇喂养兔动脉粥样硬化进展的抗动脉粥样硬化作用提供了首个证据。
