水稻端粒相关序列的克隆与定位

Cloning and mapping of telomere-associated sequences from rice.

作者信息

Ashikawa I, Kurata N, Nagamura Y, Minobe Y

机构信息

Rice Genome Research Program, National Institute of Agrobiological Resources, Society for Techno-innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.

出版信息

DNA Res. 1994;1(2):67-76. doi: 10.1093/dnares/1.2.67.

DOI:10.1093/dnares/1.2.67

PMID:7584030

Abstract

We have isolated three telomere-associated sequences from rice using cassette-ligation-mediated polymerase chain reaction (PCR). Each of the obtained clones hybridized to the terminal of one or several rice chromosome arms. The telomeres recognized by the clones displayed a high level of polymorphism between two rice varieties, Nipponbare (a japonica variety) and Kasalath (an indica variety). Variability in the chromosome termini was also detected among individual F2 progeny plants, which were derived from a cross between the two rice varieties. One clone containing telomere-associated sequences was located to one end of chromosome 5, and another clone to one end of chromosome 11. For another clone, non-allelic segregation of polymorphic hybridization bands was observed between japonica and indica rice; this clone was mapped to one end of chromosome 12 in japonica and to one end of chromosome 11 in indica rice. This indicates an exchange of termini between nonhomologous chromosomes.

摘要

我们利用盒式连接介导的聚合酶链反应（PCR）从水稻中分离出了三个端粒相关序列。每个获得的克隆都与一个或几个水稻染色体臂的末端杂交。这些克隆所识别的端粒在两个水稻品种——日本晴（粳稻品种）和卡萨拉斯（籼稻品种）之间表现出高度多态性。在两个水稻品种杂交产生的F2子代植株个体中也检测到了染色体末端的变异性。一个含有端粒相关序列的克隆定位于第5号染色体的一端，另一个克隆定位于第11号染色体的一端。对于另一个克隆，在粳稻和籼稻之间观察到多态性杂交带的非等位分离；该克隆在粳稻中定位于第12号染色体的一端，在籼稻中定位于第11号染色体的一端。这表明非同源染色体之间发生了末端交换。