Mackill D J, Zhang Z, Redoña E D, Colowit P M
United States Department of Agriculture, Davis, CA, USA.
Genome. 1996 Oct;39(5):969-77. doi: 10.1139/g96-121.
Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for gene mapping in plant species. We compared the levels of polymorphism for AFLP, RAPD, and microsatellite markers on 12 japonica and 2 indica rice cultivars. For AFLPs, seven EcoRI and seven MseI primers used in 18 primer combinations generated a total of 529 bands, of which 147 were clearly polymorphic among the accessions. The 21 RAPD primers produced 103 bands of which 43 were polymorphic. For the microsatellite markers the number of alleles per locus ranged from one (1 locus) to six. All marker types gave the same classification of the rice accessions into subspecies. Within japonica cultivars, the average percent polymorphism between any two accessions was 22% for AFLP, 24% for RAPD, and 36% for microsatellite markers (monomorphic bands excluded). The average percent polymorphism between indica and japonica accessions was 65, 35, and 76%, for AFLP, RAPD, and microsatellite markers, respectively. The total number of polymorphic bands was much higher for AFLPs, averaging over eight per gel. Seven AFLP primer combinations were assayed on 80 F2 plants of an indica x japonica cross previously mapped with RFLP markers. Of 54 AFLP bands scored, 50 could be mapped to specific chromosomes, and these appeared to be distributed throughout the rice genome. This indicates that AFLPs are a promising marker for mapping important genes in rice.
扩增片段长度多态性（AFLP）已被提议作为植物物种基因图谱构建的一种有价值的工具。我们比较了12个粳稻品种和2个籼稻品种上AFLP、RAPD和微卫星标记的多态性水平。对于AFLP，18种引物组合中使用的7种EcoRI引物和7种MseI引物共产生了529条带，其中147条在各材料间明显具有多态性。21种RAPD引物产生了103条带，其中43条具有多态性。对于微卫星标记，每个位点的等位基因数从1个（1个位点）到6个不等。所有标记类型对水稻品种的亚种分类相同。在粳稻品种内，任意两个品种间的平均多态性百分比，AFLP为22%，RAPD为24%，微卫星标记为36%（不包括单态带）。籼稻和粳稻品种间的平均多态性百分比，AFLP为65%，RAPD为35%，微卫星标记为76%。AFLP的多态性条带总数要高得多，平均每块凝胶超过8条。在一个先前用RFLP标记定位的籼粳杂交的80个F2植株上检测了7种AFLP引物组合。在检测的54条AFLP条带中，50条可以定位到特定染色体上，并且这些条带似乎分布在整个水稻基因组中。这表明AFLP是水稻重要基因定位的一种有前景的标记。
