用于测定大肠杆菌肠毒素的固相放射免疫测定法。
Solid-phase radioimmunoassay method for determination of Escherichia coli enterotoxin.
作者信息
Ceska M, Grossmüller F, Effenberger F
出版信息
Infect Immun. 1978 Feb;19(2):347-52. doi: 10.1128/iai.19.2.347-352.1978.
Abstract
The development of a solid-phase radioimmunoassay procedure for the determination of Escherichia coli enterotoxin(s) is described. Radioiodinated E. coli enterotoxin with about three radioiodine atoms per toxin molecule is, by the criterion of electrophoresis, identical to the unlabeled toxin. Goat anti-E. coli-enterotoxin antibody was coupled to polystyrene tubes and served as a solified toxin binder in the reported procedure. Various conditions necessary for the optimization and standardization of the solid-phase method were established. With the help of this technique it was possible to determine E. coli enterotoxin released from a porcine E. coli strain into culture medium.
摘要
本文描述了一种用于测定大肠杆菌肠毒素的固相放射免疫分析方法的开发。经电泳标准判断，每个毒素分子含有约三个放射性碘原子的放射性碘化大肠杆菌肠毒素与未标记的毒素相同。山羊抗大肠杆菌肠毒素抗体与聚苯乙烯管偶联，并在所报道的方法中用作固化的毒素结合剂。建立了固相方法优化和标准化所需的各种条件。借助该技术，可以测定从猪大肠杆菌菌株释放到培养基中的大肠杆菌肠毒素。
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