Hagen-Mann K, Mann W
Institut VIRION, Würzburg, Germany.
Exp Clin Endocrinol Diabetes. 1995;103(3):150-5. doi: 10.1055/s-0029-1211343.
The amplification of small amounts of nucleic acids via PCR (Mullis and Faloona, 1985) has undergone a tremendous development in biology, biochemistry, clinical diagnosis, and related fields. The typical three step reaction consisting of heat denaturation, primer annealing, and primer elongation together with the advanced technology in the instrumentation of thermal cyclers has made the reaction simple and fast. The idea to use a heat stable DNA polymerase, primers, and dNTPs in order to amplify double stranded DNA molecules in an exponential manner is not the only way to produce large amounts of nucleic acids starting from a few molecules only. This paper will give an overview of some alternatives and their applications.
通过聚合酶链式反应（PCR）（穆利斯和法洛纳，1985年）对少量核酸进行扩增，在生物学、生物化学、临床诊断及相关领域取得了巨大进展。由热变性、引物退火和引物延伸组成的典型三步反应，再加上热循环仪仪器方面的先进技术，使得该反应简单又快速。利用热稳定DNA聚合酶、引物和脱氧核苷三磷酸以指数方式扩增双链DNA分子的想法，并非仅从少数分子开始产生大量核酸的唯一方法。本文将概述一些替代方法及其应用。
