Kwoh D Y, Kwoh T J
Salk Institute Biotechnology/Industrial Associates, Inc., San Diego, California.
Am Biotechnol Lab. 1990 Oct;8(13):14-25.
Currently, PCR is the standard method for target amplification because it is the oldest and most developed procedure. However, several new alternative approaches for target amplification have recently been developed. Although these new methods are at a relatively early stage of development, each has some advantages over PCR, such as greater amplification per cycle (TAS, 3SR, Q beta), isothermal reaction (3SR), or coupled amplification-mutation detection (LAR/LAS). As a result, each may eventually gain widespread use after further development.
目前，聚合酶链反应（PCR）是靶标扩增的标准方法，因为它是最古老且发展最成熟的程序。然而，最近已开发出几种用于靶标扩增的新替代方法。尽管这些新方法尚处于相对早期的开发阶段，但每种方法都比PCR具有一些优势，例如每个循环的扩增效率更高（转录介导的扩增法、自我持续序列复制、噬菌体Qβ复制酶扩增）、等温反应（自我持续序列复制）或耦合扩增-突变检测（连接酶链反应/连接介导的扩增）。因此，每种方法在进一步发展后最终可能会得到广泛应用。
