Wusteman M, Busza A, Boylan S, Hayes A, Pegg D
Department of Biology, University of York, Heslington, United Kingdom.
Cryobiology. 1995 Oct;32(5):428-35. doi: 10.1006/cryo.1995.1042.
The rate of permeation of ethylene glycol (EG) and the maximum concentration that can be tolerated without functional damage was measured in the rabbit common carotid artery. Pairs of arteries were perfused on ice, one (the control) with a high K+ balanced salt solution containing 100 mM TES (CPTES), and the other with ethylene glycol/CPTES solutions. The concentration of EG was increased in a stepwise manner in order to reduce osmotically induced changes in endothelial cell volume. The final concentration was 10, 20, or 40% EG (w/w). After exposure for 20 min, the EG was then removed at room temperature using stepwise decreasing concentrations of ethylene glycol in the presence of 3% mannitol. After this, the contractile function of the smooth muscle was tested at 37 degrees C with noradrenaline and the integrity of the endothelium was assessed structurally by vital staining and functionally by its capacity to produce endothelium-derived relaxation factor in response to administration of acetylcholine. The tissue concentration reached 8.6% after 30 min of exposure to 10% EG. The contractile function of the smooth muscle was unaffected by EG at all concentrations. There was a significant (50%) reduction in the ACh-induced relaxation of contracted arteries after exposure to 40% EG (P < 0.02) but this was not associated with any detectable loss of cells or damage to the endothelium. It was concluded that EG warrants further investigation as a cryoprotectant for blood vessels.
在兔颈总动脉中测量了乙二醇（EG）的渗透速率以及在无功能损伤情况下可耐受的最大浓度。将成对的动脉在冰上灌注，一条（对照组）用含100 mM TES的高钾平衡盐溶液（CPTES），另一条用乙二醇/ CPTES溶液。为减少渗透压诱导的内皮细胞体积变化，逐步增加EG的浓度。最终浓度为10%、20%或40%的EG（重量/重量）。暴露20分钟后，在室温下使用含3%甘露醇且浓度逐步降低的乙二醇溶液去除EG。此后，在37℃用去甲肾上腺素测试平滑肌的收缩功能，并通过活体染色从结构上评估内皮的完整性，通过给予乙酰胆碱后产生内皮衍生舒张因子的能力从功能上评估内皮的完整性。暴露于10% EG 30分钟后，组织浓度达到8.6%。所有浓度的EG均未影响平滑肌的收缩功能。暴露于40% EG后，乙酰胆碱诱导的收缩动脉舒张有显著（50%）降低（P < 0.02），但这与任何可检测到的细胞损失或内皮损伤无关。得出的结论是，EG作为血管冷冻保护剂值得进一步研究。
