Xu H, Tian X, Li F
First Teaching Hospital, Norman Bethune University of Medical Sciences, Changchun.
Zhonghua Fu Chan Ke Za Zhi. 1995 Jul;30(7):414-6.
To study the relationship between uterine bleeding with intrauterine device (IUD) and virus infection.
Virus isolation from endometrium samples both with IUD and without IUD was performed with rabbit kidney cell. The cell pathogen effect (CPE) directed the positive isolation. All isolated viruses were further identified by polymerase chain reaction (PCR) for human cytomegalovirus (HCMV) and by neutralizing test for herpes simplex virus type II (HSV-II).
The endometrial samples were obtained from four groups: 113 patients with uterine bleeding with IUD; 109 healthy women, without uterine bleeding with IUD; 24 healthy women without IUD and 11 patients with uterine bleeding without IUD. The rates of virus isolation in four groups mentioned above were 59.29%, 19.27%, 16.67% and 63.64% respectively. The positive rates for HCMV and HSV-II in the four groups were 38.81%, 19.05%, 1/4, 3/7 and 23.88%, 23.87%, 1/4, 0.00% respectively. The unidentified viruses were 37.31%, 57.14%, 2/4 and 4/7 respectively.
These results showed that some endometrial samples from both the bleeding groups and the controls were infected by HCMV and HSV-II. The incidence of virus infection in women with uterine bleeding with IUD was much higher than that of the controls, but similar to that is the patients with uterine bleeding without IUD. The results suggested that IUD could activate latent viruses (HCMV, HSV-II) infection and induce bleeding in certain conditions.
研究宫内节育器（IUD）所致子宫出血与病毒感染之间的关系。
采用兔肾细胞对有IUD和无IUD的子宫内膜样本进行病毒分离。细胞病变效应（CPE）指导阳性分离。所有分离出的病毒通过聚合酶链反应（PCR）进一步鉴定人巨细胞病毒（HCMV），并通过中和试验鉴定Ⅱ型单纯疱疹病毒（HSV-II）。
子宫内膜样本取自四组：113例带IUD子宫出血患者；109例带IUD无子宫出血的健康女性；24例无IUD的健康女性和11例无IUD子宫出血患者。上述四组的病毒分离率分别为59.29%、19.27%、16.67%和63.64%。四组中HCMV和HSV-II的阳性率分别为38.81%、19.05%、1/4、3/7和23.88%、23.87%、1/4、0.00%。未鉴定出的病毒分别为37.31%、57.14%、2/4和4/7。
这些结果表明，出血组和对照组的一些子宫内膜样本均感染了HCMV和HSV-II。带IUD子宫出血女性的病毒感染发生率远高于对照组，但与无IUD子宫出血患者相似。结果提示，IUD在某些情况下可激活潜伏病毒（HCMV、HSV-II）感染并导致出血。
