Yasui Y, Tatsumi N, Park K, Koezuka T
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Cytometry. 1995 Mar 15;22(1):75-9. doi: 10.1002/cyto.990220114.
A flow cytometer for the automated analysis of urinary sediment was designed, and its performance was examined by the evaluation of 821 specimens. Auramine O, a dye for DNA and RNA, was used for the staining of the sediment. Urine (5 ml or more) was processed by the instrument for sediment analysis. Conventional microscopic analysis was done for comparison. The RBC count, the WBC count, and the number of bacterial cells, epithelial cells, and casts found by the flow cytometer and by microscopy were compared. Correlation was high for all these results. The overall sensitivity, specificity, and efficiency (accuracy) in the items analyzed were 84.7%, 57.8%, and 67.2%, respectively. One hundred specimens could be analyzed by the instrument per hour. The instrument seemed useful for screening for urinary tract disorders to identify specimens that should be analyzed microscopically in routine laboratories.
设计了一种用于尿沉渣自动分析的流式细胞仪，并通过对821份标本的评估来检测其性能。使用用于DNA和RNA的染料金胺O对沉渣进行染色。仪器对5毫升或更多的尿液进行沉渣分析处理。进行传统显微镜分析以作比较。比较了流式细胞仪和显微镜检查发现的红细胞计数、白细胞计数以及细菌细胞、上皮细胞和管型的数量。所有这些结果的相关性都很高。在所分析项目中的总体敏感性、特异性和效率（准确性）分别为84.7%、57.8%和67.2%。该仪器每小时可分析100份标本。该仪器似乎有助于筛查泌尿系统疾病，以识别常规实验室中应进行显微镜分析的标本。
