Yu S, Zhang Y
Department of Macrobiology, Shanghai University of TCM.
Zhonghua Zhong Liu Za Zhi. 1995 Jul;17(4):275-8.
Achyranthes bidentata polysaccharides (ABP), extracted from the root of Achyranthes bidentata Blume, 25-100mg.kg-1.d-1 x 7 could inhibit tumor growth by 31%-40%. Combination of cyclophosphamide (Cy) and ABP increased the rate of tumor growth inhibition to 58%. ABP 50 and 100% mg/kg ip could potentiate LAK cell activity and increase the Con A (5 micrograms/ml)-induced production of tumor necrosis factor (TNF-beta) from murine splenocytes. The optimal time for TNF production was on d 8. We also found that ABP 1-2 micrograms/ml strongly inhibited the proliferation of S180 and K562 cells in vitro. The S180 cell membrane content of sialic acid was increased and phospholipid decreased after ABP acting on cells for 24 hours. The changes were significantly different from the control group (P < 0.05 or P < 0.01), but the membrane cholesterol content and membrane mobility indices (Ch/PI) were not affected. The results suggest that the antitumor mechanism of ABP may be related to potentiation of the host immunosurveillance mechanism and the changes in cell membrane features.
牛膝多糖（ABP），从牛膝的根部提取，25 - 100mg·kg⁻¹·d⁻¹×7天可使肿瘤生长抑制31% - 40%。环磷酰胺（Cy）与ABP联合使用可使肿瘤生长抑制率提高到58%。ABP 50和100mg/kg腹腔注射可增强LAK细胞活性，并增加刀豆蛋白A（5μg/ml）诱导的小鼠脾细胞肿瘤坏死因子（TNF-β）的产生。TNF产生的最佳时间是第8天。我们还发现，ABP 1 - 2μg/ml在体外强烈抑制S180和K562细胞的增殖。ABP作用细胞24小时后，S180细胞膜唾液酸含量增加，磷脂减少。这些变化与对照组有显著差异（P < 0.05或P < 0.01），但膜胆固醇含量和膜流动性指数（Ch/PI）不受影响。结果表明，ABP的抗肿瘤机制可能与增强宿主免疫监视机制以及细胞膜特征的改变有关。
