Römer I, Jungblut P, Reik W, Otto A, Klose J
Institut für Toxikologie und Embryopharmakologie, Freie Universität Berlin, Germany.
Electrophoresis. 1995 May;16(5):823-30. doi: 10.1002/elps.11501601137.
A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two-dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL/6Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N-terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: alpha-enolase, cyclophilin and beta-group hemoglobins. The parental effects observed for alpha-enolase and cyclophilin were found to be due to parent-specific post-translational modifications of these proteins. For the beta-group hemoglobins our results suggested parental effects on the transcriptional level.
本文提出了一种鉴定揭示小鼠母系或父系遗传蛋白质的新策略。利用二维电泳，我们研究了C57BL/6Crl和DBA/2Crl小鼠以及它们的正反交杂种成年肝脏、不同胚胎和胚外组织中的蛋白质表达模式。我们发现了三组显示数量变异母系或父系遗传的蛋白质斑点。通过N端或内部氨基酸测序、氨基酸组成测定、糖蛋白染色和RNA表达分析对这些蛋白质进行了表征。鉴定出的三种蛋白质为：α-烯醇化酶、亲环蛋白和β-珠蛋白。观察到的α-烯醇化酶和亲环蛋白的亲本效应是由于这些蛋白质的亲本特异性翻译后修饰。对于β-珠蛋白，我们的结果表明亲本对转录水平有影响。
