Ozaki K, Fujiwara T, Kawai A, Shimizu F, Takami S, Okuno S, Takeda S, Shimada Y, Nagata M, Watanabe T, Takaichi A, Takahashi E, Nakamura Y, Shin S
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
Cytogenet Cell Genet. 1996;72(2-3):242-5. doi: 10.1159/000134199.
We isolated a human cDNA clone encoding a novel protein homologous to cyclophilins, specific cellular targets of cyclosporin A, which are conserved in species ranging from human to prokaryotes. This cDNA, designated hCyPX, contained an open reading frame of 498 nucleotides encoding 166 amino acids. Computer analysis indicated that its predicted amino acid sequence had 41.6%, 40.4%, and 39.2% homology to those of human, bovine, and Drosophila cyclophilins, respectively. Northern blot analysis indicated ubiquitous expression in adult human tissues, but most abundant expression in heart. Fluorescence in situ hybridization to human metaphase chromosomes localized this gene (PPIL1, peptidylprolyl isomerase [cyclophilin]-like 1) to chromosome bands 2p23.3-->p23.1.
我们分离出一个人类cDNA克隆，它编码一种与亲环蛋白同源的新型蛋白质，亲环蛋白是环孢菌素A的特定细胞靶点，在从人类到原核生物的物种中都保守存在。这个被命名为hCyPX的cDNA包含一个498个核苷酸的开放阅读框，编码166个氨基酸。计算机分析表明，其预测的氨基酸序列分别与人类、牛和果蝇的亲环蛋白具有41.6%、40.4%和39.2%的同源性。Northern印迹分析表明该基因在成人组织中普遍表达，但在心脏中表达最为丰富。对人类中期染色体进行荧光原位杂交，将该基因（PPIL1，肽基脯氨酰异构酶[亲环蛋白]样1）定位到染色体带2p23.3→p23.1。
