Friedman J, Weissman I, Friedman J, Alpert S
Department of Pathology and Developmental Biology, Stanford University School of Medicine, CA 94305.
Am J Pathol. 1994 Jun;144(6):1247-56.
Cyclophilin C (cyp C) is a cyclosporin A (CsA) binding protein originally isolated from a mouse bone marrow stromal cell line. We have compared the expression patterns of the mammalian cyclophilins A, B, and C in mouse tissues using in situ hybridization. These studies reveal that cyp C is expressed in a restricted subset of tissues including mouse ovary, testis, bone marrow, and kidney. Within the kidney, cyp C is highly expressed in a narrow zone in the outer medulla. Using monoclonal antibodies reactive against cyp C, we find that the kidney cells expressing cyp C correspond to the S3 segment of the nephron. The S3 segment has been shown to sustain histopathological damage from high dosages of CsA, raising the possibility that cyp C may be involved in mediating this damage.
亲环蛋白C（cyp C）是一种最初从小鼠骨髓基质细胞系中分离出来的环孢素A（CsA）结合蛋白。我们使用原位杂交技术比较了哺乳动物亲环蛋白A、B和C在小鼠组织中的表达模式。这些研究表明，cyp C在包括小鼠卵巢、睾丸、骨髓和肾脏在内的有限组织亚群中表达。在肾脏中，cyp C在外髓质的一个狭窄区域高度表达。使用针对cyp C的单克隆抗体，我们发现表达cyp C的肾细胞对应于肾单位的S3段。S3段已被证明会受到高剂量CsA的组织病理学损伤，这增加了cyp C可能参与介导这种损伤的可能性。
