Marin M G, Lillo F, Varnier O E, Bresciani S, Molinelli A, Abecasis C, Bonini P A, Albertini A
Institute of Chemistry, School of Medicine, University of Brescia, Spedali Civili, Italy.
Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):621-5. doi: 10.1007/BF01690739.
The performance and clinical relevance of a qualitative PCR-based assay for the detection of HIV-1 DNA sequences in peripheral blood mononuclear cells (PBMCs) was evaluated by two different laboratories. Four hundred and one samples were obtained from 397 individuals from different risk populations. All blood donors tested had negative results; positive signals were obtained from all infected patients. HIV-1 DNA was detected in 3 of 17 infants born to seropositive mothers; Western blot indeterminate blood donors and exposed health-care workers had negative results. Our results demonstrate that this PCR assay provides both sensitive and specific results and is suitable for testing large numbers of samples and for rapid identification of HIV-1 infection.
两个不同实验室评估了一种基于定性聚合酶链反应（PCR）的检测外周血单个核细胞（PBMCs）中HIV-1 DNA序列的检测方法的性能及其临床相关性。从397名来自不同风险人群的个体中获取了401份样本。所有接受检测的献血者结果均为阴性；所有感染患者均获得阳性信号。在17名血清反应阳性母亲所生的婴儿中，有3名检测到HIV-1 DNA；免疫印迹结果不确定的献血者和接触过HIV的医护人员检测结果为阴性。我们的结果表明，这种PCR检测方法能提供灵敏且特异的结果，适用于检测大量样本以及快速鉴定HIV-1感染。
