Waterfield C J, Jairath M, Asker D S, Timbrell J A
Department of Toxicology, School of Pharmacy, University of London, UK.
Eur J Pharmacol. 1995 Jul 1;293(2):141-9. doi: 10.1016/0926-6917(95)00010-0.
Administration of clenbuterol to rats in the drinking water over a 4 day period increased incorporation of [3H]leucine into muscle protein and caused a slight reduction in urinary 3-methylhistidine but did not result in an increase in body or muscle weight. However, both urinary and liver taurine were significantly reduced at the highest dose of clenbuterol (2 mg.kg-1.day-1). Serum creatine kinase, muscle isoenzyme (CK-MM) was raised and single muscle fibre injury was observed in the soleus muscle in animals treated with the middle dose (0.2 mg.kg-1.day-1) and highest dose (2 mg.kg-1.day-1). The reduction in the body pool of taurine caused by clenbuterol is of concern as taurine has been shown to have protective properties.
在4天的时间里，给大鼠饮用含克伦特罗的水，可增加[3H]亮氨酸掺入肌肉蛋白的量，并使尿中3-甲基组氨酸略有减少，但并未导致体重或肌肉重量增加。然而，在克伦特罗最高剂量（2毫克·千克-1·天-1）时，尿中和肝脏中的牛磺酸均显著减少。用中剂量（0.2毫克·千克-1·天-1）和最高剂量（2毫克·千克-1·天-1）处理的动物，血清肌酸激酶、肌肉同工酶（CK-MM）升高，并且在比目鱼肌中观察到单根肌纤维损伤。由于牛磺酸已被证明具有保护作用，因此克伦特罗导致体内牛磺酸池减少令人担忧。
