Kaufman R A, Menezo Y, Hazout A, Nicollet B, DuMont M, Servy E J
Augusta Reproductive Biology Associates, Georgia 30904, USA.
Fertil Steril. 1995 Dec;64(6):1125-9.
To present our experience using cocultured cryopreserved and transferred blastocysts.
Retrospective study of patients undergoing transfer of cryopreserved blastocysts.
Three different IVF centers.
Four hundred sixty-seven thawed cycles from January 1991 to June 1994.
Pregnancy rate per cycle after transfer of pre-embryos developed from thawed blastocysts.
One thousand two hundred thirty-nine blastocysts were thawed. Of these, 1,033 (83%) survived thawing and were transferred. Five hundred sixty-three thawed cycles resulted in 516 (92%) receiving intrauterine transfer. One hundred twelve clinical pregnancies were established, resulting in a 21.7% pregnancy per transfer with a 19% ongoing rate. The implantation rate of 13.4% results from 138 implanted pre-embryos. There was a higher PR in the programmed cycle (79/302; 26.2%) compared with the natural cycle (6/47;13%).
Freezing at the blastocyst stage is a proven and reliable method in IVF technology. Although there may be fewer pre-embryos, their ability to implant appears to approach the potential of a fresh transfer.
介绍我们使用共培养冷冻保存并移植囊胚的经验。
对接受冷冻囊胚移植患者的回顾性研究。
三个不同的体外受精中心。
1991年1月至1994年6月的467个解冻周期。
解冻囊胚发育而来的胚胎移植后每个周期的妊娠率。
共解冻1239个囊胚。其中，1033个（83%）解冻后存活并进行了移植。563个解冻周期中有516个（92%）接受了子宫内移植。确立了112例临床妊娠，每次移植的妊娠率为21.7%，持续妊娠率为19%。138个着床胚胎的着床率为13.4%。与自然周期（6/47；13%）相比，计划周期的妊娠率更高（79/302；26.2%）。
囊胚期冷冻是体外受精技术中一种经过验证且可靠的方法。虽然胚胎数量可能较少，但其着床能力似乎接近新鲜移植的潜力。
