Leung F Y, Henderson A R
Clin Chem. 1979 Feb;25(2):209-11.
We assessed the clinical efficacy of a thin-layer agarose electrophoresis assay for lactate dehydrogenase isoenzyme estimation in the diagnosis of acute myocardial infarction. From a population of 228 patients admitted to the Coronary Care Unit with suspected acute myocardial infarction, all 101 positive cases (confirmed by clinical presentation and electrocardiographic changes) were correctly identified with lactate dehydrogenase isoenzyme-1 percentage of total activity above the reference range and with a lactate dehydrogenase isoenzyme-1/isoenzyme-2 ratio of 0.76 or above. This ratio was between 0.45 and 0.74 for 250 healthy subjects. No falsely negative, but 12 falsely positive results were obtained from this Coronary Care Unit population, to give a sensitivity of 100% and a specificity of 90.5% for the ratio test.
我们评估了薄层琼脂糖电泳法测定乳酸脱氢酶同工酶在急性心肌梗死诊断中的临床疗效。在228名入住冠心病监护病房且疑似急性心肌梗死的患者中，所有101例阳性病例（通过临床表现和心电图变化确诊）的乳酸脱氢酶同工酶-1占总活性的百分比均高于参考范围，且乳酸脱氢酶同工酶-1/同工酶-2比值为0.76或更高，均被正确识别。250名健康受试者的该比值在0.45至0.74之间。从该冠心病监护病房人群中未获得假阴性结果，但有12例假阳性结果，该比值检测的灵敏度为100%，特异性为90.5%。
