Isaacson R E, Trigo E
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801, USA.
FEMS Microbiol Lett. 1995 Oct 15;132(3):247-51. doi: 10.1016/0378-1097(95)00317-x.
Using electron microscopy, pili with at least two distinct morphologies were observed on strains of Pasteurella multocida isolated from pigs with atrophic rhinitis. Rigid pili were found on 60-80% of all cells observed. These pili had a strong tendency to lie flat along the side of the outer cell membrane of P. multocida and as a result frequently were difficult to see. After growth in vitro, piliated P. multocida cells produced few pili (approx. 3-5 per cell). Heavily piliated cells were occasionally observed. The second type of pili were curly and also were difficult to visualize. Cells from cultures containing piliated cells failed to attach to red blood cells and to immobilized mucus.
利用电子显微镜,在从患有萎缩性鼻炎的猪身上分离出的多杀性巴氏杆菌菌株上观察到了至少两种不同形态的菌毛。在所观察的所有细胞中,60% - 80%的细胞上发现了刚性菌毛。这些菌毛有强烈的沿着多杀性巴氏杆菌外细胞膜一侧平躺的趋势,因此常常很难看到。体外培养后,有菌毛的多杀性巴氏杆菌细胞产生的菌毛很少(每个细胞约3 - 5根)。偶尔会观察到菌毛很多的细胞。第二种菌毛是卷曲的,同样难以观察到。含有有菌毛细胞的培养物中的细胞不能附着于红细胞和固定化的黏液。