Il'ina T S, Fil'kova S L
Genetika. 1995 Jul;31(7):877-82.
The large pVM82 plasmid isolated from epidemic strains of Yersinia pseudotuberculosis includes the 25MD segment, which encodes a series of properties affecting the virulence of the bacterium. Insertion mutants of pVM82 containing transposition-defective Tn2507 with a kanamycin-resistance marker in different Hind III fragments of the 25MD segment were obtained. By recombination between two homologous pVM82 containing genetic markers in different parts, deletion derivatives of pVM82 plasmid and insertions of the plasmid segment, carrying kanamycin-resistance marker, into a chromosome were obtained. Results were obtained suggesting the presence in the plasmid 25MD segment of a transposon-like structure capable of migrating from pVM82 plasmid onto a chromosome and from a chromosome and pVM82 onto pRP1.2 plasmid of a broad host range.
从假结核耶尔森氏菌流行菌株中分离出的大质粒pVM82包含25MD片段，该片段编码一系列影响细菌毒力的特性。获得了在25MD片段的不同Hind III片段中含有带有卡那霉素抗性标记的转座缺陷型Tn2507的pVM82插入突变体。通过在不同部位含有遗传标记的两个同源pVM82之间的重组，获得了pVM82质粒的缺失衍生物以及携带卡那霉素抗性标记的质粒片段插入染色体的产物。结果表明，在质粒25MD片段中存在一种转座子样结构，它能够从pVM82质粒迁移到染色体上，也能从染色体和pVM82迁移到广宿主范围的pRP1.2质粒上。
