Fujita T, Sakuma S, Fujimoto K, Yoshioka K, Ashida E, Nishida H, Fujimoto Y
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Free Radic Biol Med. 1995 Oct;19(4):487-91. doi: 10.1016/0891-5849(95)00029-w.
Effects of various fatty acids and fatty acyl CoA esters on Cu(2+)-induced conversion of xanthine dehydrogenase to oxidase in rabbit liver were examined. Cu2+ (2-10 microM) brought about the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. Oleic, arachidonic, eicosapentaenoic, and docosahexaenoic acids (50-200 microM) prevented the conversion of xanthine dehydrogenase to oxidase catalyzed by 6 microM-Cu2+. The effect of these four fatty acids was concentration-dependent, whereas palmitic, stearic, and linoleic acids had no effect on the conversion of xanthine dehydrogenase to oxidase at the same concentration range. On the other hand, palmitoyl, linoleoyl, and arachidonoyl CoAs elicited the inhibition of 6 microM-Cu(2+)-induced conversion of xanthine dehydrogenase to oxidase at concentrations of 50, 100, and 200 microM. These results suggest that oleic, arachidonic, eicosapentaenoic and docosahexaenoic acids, and fatty acyl CoAs have the potential to inhibit the conversion of xanthine dehydrogenase to oxidase in rabbit liver.
研究了各种脂肪酸和脂肪酰辅酶A酯对兔肝中铜（2+）诱导的黄嘌呤脱氢酶向氧化酶转化的影响。铜离子（2 - 10微摩尔）以剂量依赖的方式导致黄嘌呤脱氢酶向氧化酶的转化。油酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸（50 - 200微摩尔）可阻止由6微摩尔铜离子催化的黄嘌呤脱氢酶向氧化酶的转化。这四种脂肪酸的作用呈浓度依赖性，而棕榈酸、硬脂酸和亚油酸在相同浓度范围内对黄嘌呤脱氢酶向氧化酶的转化没有影响。另一方面，棕榈酰辅酶A、亚油酰辅酶A和花生四烯酰辅酶A在浓度为50、100和200微摩尔时可抑制6微摩尔铜（2+）诱导的黄嘌呤脱氢酶向氧化酶的转化。这些结果表明，油酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸以及脂肪酰辅酶A有潜力抑制兔肝中黄嘌呤脱氢酶向氧化酶的转化。
