铜离子、锌离子和硒离子诱导兔肝中黄嘌呤脱氢酶向氧化酶的转化

Induction of the conversion of xanthine dehydrogenase to oxidase in rabbit liver by Cu2+,Zn2+ and selenium ions.

作者信息

Sakuma S, Fujimoto Y, Iwai M, Fujimoto K, Yasuda M, Nishida H, Fujita T

机构信息

Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.

出版信息

J Pharm Pharmacol. 1994 Jun;46(6):487-90. doi: 10.1111/j.2042-7158.1994.tb03832.x.

DOI:10.1111/j.2042-7158.1994.tb03832.x

PMID:7932044

Abstract

Effects of Cu2+,Zn2+,Fe2+ and selenium ions on the conversion of xanthine dehydrogenase to oxidase in rabbit liver were examined. Under basal conditions, xanthine oxidase activity represented only 16% of the total xanthine oxidase plus dehydrogenase activity. Cu2+ (2-10 microM), Zn2+ (5-30 microM) and selenium ions (5-100 microM) brought about the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. The concentrations of Cu2+,Zn2+ and selenium ions required for increasing xanthine oxidase activity by 50% was approximately 4, 10 and 20 microM, respectively. On the other hand, Fe2+ had no effect on the conversion of the enzyme up to 100 microM. These results suggest that Cu2+,Zn2+ and selenium ions have the potential to modulate the conversion of xanthine dehydrogenase to oxidase in rabbit liver.

摘要

研究了铜离子（Cu2+）、锌离子（Zn2+）、亚铁离子（Fe2+）和硒离子对兔肝脏中黄嘌呤脱氢酶向氧化酶转化的影响。在基础条件下，黄嘌呤氧化酶活性仅占黄嘌呤氧化酶与脱氢酶总活性的16%。铜离子（2 - 10微摩尔/升）、锌离子（5 - 30微摩尔/升）和硒离子（5 - 100微摩尔/升）以剂量依赖的方式促使黄嘌呤脱氢酶转化为氧化酶。使黄嘌呤氧化酶活性增加50%所需的铜离子、锌离子和硒离子浓度分别约为4、10和20微摩尔/升。另一方面，高达100微摩尔/升的亚铁离子对该酶的转化没有影响。这些结果表明，铜离子、锌离子和硒离子有可能调节兔肝脏中黄嘌呤脱氢酶向氧化酶的转化。