Yip S P, Yow C M, Lewis W H
Department of Health Sciences, Hong Kong Polytechnic University, Kowloon.
Hum Hered. 1995 Sep-Oct;45(5):266-71. doi: 10.1159/000154311.
DNA polymorphism at the ABO locus was investigated using denaturing gradient gel electrophoresis of polymerase-chain-reaction-amplified DNA products from 315 healthy individuals of the Chinese population of Hong Kong. Five different alleles were identified: ABOO1, O2, A1, A2 and B1. The ABOA2 is a new allele not previously reported in the literature. The allele frequencies found were 0.3698 for O1, 0.2508 for O2, 0.1952 for A1, 0.0159 for A2, and 0.1683 for B1. The Chinese population being investigated was in Hardy-Weinberg equilibrium. The genotyping method identifies many more alleles in the ABO locus and thus makes it a more useful genetic marker in linkage analysis, paternity testing and individualization in forensic work than when the locus is genotyped using conventional DNA-based methods or phenotyped using serological methods.
利用聚合酶链反应扩增来自315名香港中国健康人群的DNA产物，通过变性梯度凝胶电泳研究ABO位点的DNA多态性。鉴定出五个不同的等位基因：ABOO1、O2、A1、A2和B1。ABOA2是一个文献中此前未报道过的新等位基因。所发现的等位基因频率分别为：O1为0.3698，O2为0.2508，A1为0.1952，A2为0.0159，B1为0.1683。所研究的中国人群处于哈迪-温伯格平衡。与使用传统基于DNA的方法对该位点进行基因分型或使用血清学方法进行表型分型相比，这种基因分型方法能鉴定出ABO位点更多的等位基因，因此使其在连锁分析、亲子鉴定和法医工作中的个体识别方面成为更有用的遗传标记。
