将包含糖蛋白III基因的2.4 kb牛疱疹病毒1型DNA片段克隆到pUC18质粒载体中。
Cloning of 2.4 kb bovine herpesvirus-1 DNA fragment containing glycoprotein III gene into pUC18 plasmid vector.
作者信息
Gupta P K, Chaudhuri P, Sharma B, Singh V P, Balain D S
机构信息
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India.
出版信息
Indian J Exp Biol. 1995 Jun;33(6):459-61.
A recombinant pBR322 plasmid containing bovine herpesvirus-1 HindIII 'I' fragment was analysed using EcoRI and BamHI restriction endonucleases. This recombinant plasmid was labelled with [alpha 32P]dATP and hybridized with southern blot of HindIII digested BHV-1 DNA fragments. A 2.4 kb double digested EcoRI-BamHI fragment of HindIII 'I' was subcloned into pUC18 plasmid to get complete gIII gene. The recombinant pUC18 plasmid was analysed for 2.4 kb BHV-1 DNA insert by restriction digestion with EcoRI and BamHI. Southern blot of restriction digested plasmid was hybridized with [alpha 32P]dATP labelled BHV-1 DNA probe.
使用EcoRI和BamHI限制性内切酶分析了含有牛疱疹病毒1型HindIII‘I’片段的重组pBR322质粒。该重组质粒用[α-32P]dATP进行标记，并与经HindIII消化的BHV-1 DNA片段的Southern印迹杂交。将HindIII‘I’的一个2.4 kb双酶切EcoRI-BamHI片段亚克隆到pUC18质粒中，以获得完整的gIII基因。通过用EcoRI和BamHI进行限制性消化，分析重组pUC18质粒中2.4 kb的BHV-1 DNA插入片段。用[α-32P]dATP标记的BHV-1 DNA探针与限制性消化质粒的Southern印迹杂交。
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