Gupta P K, Rai A
National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar.
Biochem Mol Biol Int. 1993 Apr;29(5):889-98.
An Indian isolate of BHV-1 was analyzed using several restriction endonucleases. The restriction pattern of BHV-1 DNA with HindIII, BamHI and EcoRI was found to be similar to other reported restriction endonuclease patterns for respiratory isolates of BHV-1. Thus, this isolate could be grouped under BHV-1.1 (respiratory form of BHV-1). Few restriction endonucleases yielded distinct restriction profile of BHV-1 DNA. The total molecular size of BHV-1 DNA for the isolate studied was also estimated to be within 136 kb to 138 kb.
使用几种限制性内切酶对一株印度的牛疱疹病毒1型(BHV-1)分离株进行了分析。发现BHV-1 DNA用 HindIII、BamHI 和 EcoRI 酶切后的图谱与其他报道的BHV-1呼吸道分离株的限制性内切酶图谱相似。因此,该分离株可归类为BHV-1.1(BHV-1的呼吸道形式)。少数限制性内切酶产生了BHV-1 DNA的独特酶切图谱。所研究的该分离株的BHV-1 DNA的总分子大小估计也在136 kb至138 kb之间。