Malven P V, Haglof S A, Jiang H
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
J Anim Sci. 1995 Jul;73(7):2105-12. doi: 10.2527/1995.7372105x.
Ovine antiserum against a conjugate of porcine neuropeptide Y (NPY) with bovine thyroglobulin was infused repeatedly into one lateral ventricle of five ovariectomized (ovx) ewes before and after subcutaneous injection of estradiol-17 beta (E beta). Serum concentrations of LH, growth hormone (GH), and prolactin (PRL) were measured at 10-min intervals before E beta and at approximately hourly intervals 8 to 18 h after E beta injection. Control ewes were infused with ovine serum from animals immunized against bovine thyroglobulin. Basal episodic profiles (pre-E beta) of LH, GH, and PRL were similar in ovx ewes infused with control or anti-NPY serum. Injection of E beta induced a surge-like increase of serum LH, which began at 12.6 h after E beta in ewes infused with anti-NPY and at 14.4 h after E beta in ewes infused with control antiserum (P < .05). The magnitude of the E beta-induced surge of LH was not different between treatments. In addition to initiating a surge-like release of LH over the period 12 to 18 h after E beta, serum GH was transiently increased during the period between 10 and 15 h after E beta. In contrast, serum PRL was increased during the entire period between 8 and 18 h after E beta. Based on the effects of immunoneutralization, endogenous NPY in the brain of E beta-treated ovx ewes seems to restrain or delay the onset of the surge-like secretion of LH and probably GnRH, but endogenous NPY does not affect the episodic pulsatile releases of LH characteristic of ovx ewes.(ABSTRACT TRUNCATED AT 250 WORDS)
在皮下注射17β-雌二醇（Eβ）前后，将针对猪神经肽Y（NPY）与牛甲状腺球蛋白偶联物的绵羊抗血清反复注入5只去卵巢（ovx）母羊的一侧脑室。在注射Eβ前每隔10分钟测量一次血清促黄体生成素（LH）、生长激素（GH）和催乳素（PRL）的浓度，在注射Eβ后8至18小时每隔约1小时测量一次。对照母羊注入针对牛甲状腺球蛋白免疫的动物的绵羊血清。注入对照或抗NPY血清的ovx母羊中，LH、GH和PRL的基础脉冲式分泌模式（注射Eβ前）相似。注射Eβ诱导血清LH呈激增样升高，在注入抗NPY的母羊中，Eβ注射后12.6小时开始，在注入对照抗血清的母羊中，Eβ注射后14.4小时开始（P<0.05）。不同处理之间，Eβ诱导的LH激增幅度没有差异。除了在Eβ注射后12至18小时引发LH的激增样释放外，血清GH在Eβ注射后10至15小时短暂升高。相比之下，血清PRL在Eβ注射后8至18小时的整个时间段内都升高。基于免疫中和的作用，Eβ处理的ovx母羊大脑中的内源性NPY似乎抑制或延迟了LH以及可能还有GnRH激增样分泌的起始，但内源性NPY不影响ovx母羊特有的LH的脉冲式释放。（摘要截短至250字）
