Hickner R C, Ekelund U, Mellander S, Ungerstedt U, Henriksson J
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
J Appl Physiol (1985). 1995 Aug;79(2):638-47. doi: 10.1152/jappl.1995.79.2.638.
A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0 microliter/min were employed. However, a linear decrease was found between 4 and approximately 45 ml.100 g-1.min-1 (r = -0.92 to -0.99). The lower outflow-to-inflow ratio was at 4 ml.100 g-1.min-1 (i.e., due to a low probe perfusion flow rate or a large dialysis membrane), the greater the sensitivity of the method was. It is concluded that this nonradioactive technique provides a simple and valid method for determining nutritive blood flow in skeletal muscle.
在猫的腓肠肌中对微透析乙醇技术进行了定量验证。将6至8个微透析探针插入离体肌肉标本中，并用含有5至1000 mmol/l乙醇的克雷布斯 - 亨塞尔特缓冲液进行灌注（0.5 - 10.0微升/分钟）。通过伺服控制的滚轮泵使骨骼肌血流量在4 - 99 ml·100 g⁻¹·min⁻¹范围内保持恒定，并使用微透析乙醇技术以及通过定时收集静脉流出液来测定血流量。当采用0.5和1.0微升/分钟的微透析灌注流速时，乙醇浓度流出/流入比（[乙醇]收集的透析液/[乙醇]注入的灌注介质）呈非线性下降。然而，在4至约45 ml·100 g⁻¹·min⁻¹之间发现呈线性下降（r = -0.92至 -0.99）。在4 ml·100 g⁻¹·min⁻¹时流出/流入比越低（即由于探针灌注流速低或透析膜大），该方法的灵敏度越高。结论是，这种非放射性技术为测定骨骼肌中的营养性血流量提供了一种简单有效的方法。
